ArticleJournal of bacteriology2010
The threshold level of the sensor histidine kinase KinA governs entry into sporulation in Bacillus subtilis.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 72 citations in OpenAlex.
- Article
- Conservation of sporulation genes and a transmembrane-containing Spo0B variant inbioRxiv : the preprint server for biology · 2025Article
- Systematic modulation of bacterial resource allocation by perturbing RNA polymerase availability via synthetic transcriptional switches.Nucleic acids research · 2025Article
- Biophysical modeling reveals the transcriptional regulatory mechanism of Spo0A, the master regulator in starvingmSystems · 2025Article
- Unique growth and morphology properties of Clade 5bioRxiv : the preprint server for biology · 2025Article
- Unique growth and morphology properties of Clade 5 Clostridioides difficile strains revealed by single-cell time-lapse microscopy.PLoS pathogens · 2025Article
- Phosphorelay changes and plasticity underlie the life history evolution ofMicrobiology (Reading, England) · 2025Article
- Altruistic feeding and cell-cell signaling during bacterial differentiation actively enhance phenotypic heterogeneity.Science advances · 2024Article
- Inactivation of guanylate kinase inHeliyon · 2024Article
- Review
- Altruistic feeding and cell-cell signaling during bacterial differentiation actively enhance phenotypic heterogeneity.bioRxiv : the preprint server for biology · 2024Article
- Termination factor Rho mediates transcriptional reprogramming of Bacillus subtilis stationary phase.PLoS genetics · 2023Article
- Kinase expression enhances phenolic aldehydes conversion and ethanol fermentability of Zymomonas mobilis.Bioprocess and biosystems engineering · 2022Article
- Article
- A new role for SR1 from Bacillus subtilis: regulation of sporulation by inhibition of kinA translation.Nucleic acids research · 2021Article
- Advances in engineeredSynthetic and systems biotechnology · 2021Review
- High Resolution Analysis of Proteome Dynamics duringInternational journal of molecular sciences · 2021Article
- Article
- Characterization of a Protease Hyper-Productive Mutant of Bacillus pumilus by Comparative Genomic and Transcriptomic Analysis.Current microbiology · 2020Article
- Synthesis of histidine kinase inhibitors and their biological properties.Medicinal research reviews · 2020Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sporulation in Bacillus subtilis is controlled by a complex gene regulatory circuit that is activated upon nutrient deprivation. The initial process is directed by the phosphorelay, involving the major sporulation histidine kinase (KinA) and two additional phosphotransferases (Spo0F and Spo0B), that activates the master transcription factor Spo0A. Little is known about the initial event and mechanisms that trigger sporulation. Using a strain in which the synthesis of KinA is under the control of an IPTG (isopropyl-beta-d-thiogalactopyranoside)-inducible promoter, here we demonstrate that inducing the synthesis of the KinA beyond a certain level leads to the entry of the irreversible process of sporulation irrespective of nutrient availability. Moreover, the engineered cells expressing KinA under a sigma(H)-dependent promoter that is similar to but stronger than the endogenous kinA promoter induce sporulation during growth. These cells, which we designated COS (constitutive sporulation) cells, exhibit the morphology and properties of sporulating cells and express sporulation marker genes under nutrient-rich conditions. Thus, we created an engineered strain displaying two cell cycles (growth and sporulation) integrated into one cycle irrespective of culture conditions, while in the wild type, the appropriate cell fate decision is made depending on nutrient availability. These results suggest that the threshold level of the major sporulation kinase acts as a molecular switch to determine cell fate and may rule out the possibility that the activity of KinA is regulated in response to the unknown signal(s).
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