ArticleJournal of bacteriology2010
Role of MrkJ, a phosphodiesterase, in type 3 fimbrial expression and biofilm formation in Klebsiella pneumoniae.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- The dual role of cAMP receptor protein (CRP) in regulating type 3 fimbriae expression in Klebsiella pneumoniae CG43S3 in response to oxygen availability.BMC microbiology · 2026Article
- Article
- Rapid evolution of Klebsiella pneumoniae biofilms in vitro delineates adaptive changes selected during infection.Nature communications · 2026Article
- The Nucleoid-Associated Protein Fis Represses Type 3 Fimbriae to Modulate Biofilm and Adherence Formation inMicroorganisms · 2025Article
- Regulation of type 3 fimbria expression by RstA affects biofilm formation and virulence inMicrobiology spectrum · 2025Article
- Nucleotides as Bacterial Second Messengers.Molecules (Basel, Switzerland) · 2023Review
- Widespread Detection of Yersiniabactin Gene Cluster and Its Encoding Integrative Conjugative Elements (ICEMicrobiology spectrum · 2023Article
- Virulence Characteristics and Molecular Typing of Carbapenem-Resistant ST15Antibiotics (Basel, Switzerland) · 2023Article
- Regulation of biofilm formation inFrontiers in microbiology · 2023Review
- Conserved FimK Truncation Coincides with Increased Expression of Type 3 Fimbriae and Cultured Bladder Epithelial Cell Association in Klebsiella quasipneumoniae.Journal of bacteriology · 2022Article
- Regulation of c-di-GMP in Biofilm Formation of Klebsiella pneumoniae in Response to Antibiotics and Probiotic Supernatant in a Chemostat System.Current microbiology · 2021Article
- Functional Insights From KpfR, a New Transcriptional Regulator of Fimbrial Expression That Is Crucial forFrontiers in microbiology · 2020Article
- Down-Regulation of Flagellar, Fimbriae, and Pili Proteins in Carbapenem-ResistantFrontiers in microbiology · 2019Article
- Exploring the Links between Nucleotide Signaling and Quorum Sensing Pathways in Regulating Bacterial Virulence.ACS infectious diseases · 2018Review
- Carbapenem-Resistant Hypervirulent Klebsiella pneumoniae of Sequence Type 36.Antimicrobial agents and chemotherapy · 2018Article
- Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR.Scientific reports · 2018Article
- Pseudomonas aeruginosa and Klebsiella pneumoniae Adaptation to Innate Immune Clearance Mechanisms in the Lung.Journal of innate immunity · 2018Review
- Cyclic-di-GMP regulation of virulence in bacterial pathogens.Wiley interdisciplinary reviews. RNA · 2018Review
- Phosphorylated OmpR Is Required for Type 3 Fimbriae Expression inFrontiers in microbiology · 2018Article
- Stand-Alone EAL Domain Proteins Form a Distinct Subclass of EAL Proteins Involved in Regulation of Cell Motility and Biofilm Formation in Enterobacteria.Journal of bacteriology · 2017Article
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2 authors.
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Abstract
Klebsiella pneumoniae is an opportunistic pathogen that has been shown to adhere to human extracellular matrices using the type 3 fimbriae. Introduction of plasmids carrying genes known to alter intracellular cyclic-di-GMP pools in Vibrio parahaemolyticus revealed that these genes also altered type 3 fimbrial surface expression in K. pneumoniae. Immediately adjacent to the type 3 fimbrial gene cluster is a gene, mrkJ, that is related to a family of bacterial genes encoding phosphodiesterases. We identify here a role for MrkJ, a functional phosphodiesterase exhibiting homology to EAL domain-containing proteins, in controlling type 3 fimbria production and biofilm formation in K. pneumoniae. Deletion of mrkJ resulted in an increase in type 3 fimbria production and biofilm formation as a result of the accumulation of intracellular cyclic-di-GMP. This gene was shown to encode a functional phosphodiesterase via restoration of motility in a V. parahaemolyticus strain previously shown to accumulate cyclic-di-GMP and in vitro using phosphodiesterase activity assays. The effect of the mrkJ mutation on type 3 fimbrial expression was shown to be at the level of mrkA gene transcription by using quantitative reverse transcription-PCR. These results reveal a previously unknown role for cyclic-di-GMP in type 3 fimbrial production.
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