ArticleJournal of bacteriology2010
Replication of Vibrio cholerae chromosome I in Escherichia coli: dependence on dam methylation.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 24 citations in OpenAlex.
- The replication enhancer crtS depends on transcription factor Lrp for modulating binding of initiator RctB to ori2 of Vibrio cholerae.Nucleic acids research · 2024Article
- Cell cycle-coordinated maintenance of theEcoSal Plus · 2023Review
- Vibrio cholerae chromosome 2 copy number is controlled by the methylation-independent binding of its monomeric initiator to the chromosome 1 crtS site.Nucleic acids research · 2018Article
- Synchronous termination of replication of the two chromosomes is an evolutionary selected feature in Vibrionaceae.PLoS genetics · 2018Article
- Functionality of Two Origins of Replication inFrontiers in microbiology · 2018Article
- Replicate Once Per Cell Cycle: Replication Control of Secondary Chromosomes.Frontiers in microbiology · 2018Review
- Establishing a System for Testing Replication Inhibition of the Vibrio cholerae Secondary Chromosome in Escherichia coli.Antibiotics (Basel, Switzerland) · 2017Article
- Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression.Nucleic acids research · 2015Article
- DNA Methylation.EcoSal Plus · 2014Article
- oriC-encoded instructions for the initiation of bacterial chromosome replication.Frontiers in microbiology · 2014Review
- DNA methylation by CcrM activates the transcription of two genes required for the division of Caulobacter crescentus.Molecular microbiology · 2013Article
- Genome engineering in Vibrio cholerae: a feasible approach to address biological issues.PLoS genetics · 2012Article
- Article
- Molecular insights into replication initiation in a multipartite genome harboring bacterium Deinococcus radiodurans.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We successfully substituted Escherichia coli's origin of replication oriC with the origin region of Vibrio cholerae chromosome I (oriCI(Vc)). Replication from oriCI(Vc) initiated at a similar or slightly reduced cell mass compared to that of normal E. coli oriC. With respect to sequestration-dependent synchrony of initiation and stimulation of initiation by the loss of Hda activity, replication initiation from oriC and oriCI(Vc) were similar. Since Hda is involved in the conversion of DnaA(ATP) (DnaA bound to ATP) to DnaA(ADP) (DnaA bound to ADP), this indicates that DnaA associated with ATP is limiting for V. cholerae chromosome I replication, which similar to what is observed for E. coli. No hda homologue has been identified in V. cholerae yet. In V. cholerae, dam is essential for viability, whereas in E. coli, dam mutants are viable. Replacement of E. coli oriC with oriCI(Vc) allowed us to specifically address the role of the Dam methyltransferase and SeqA in replication initiation from oriCI(Vc). We show that when E. coli's origin of replication is substituted by oriCI(Vc), dam, but not seqA, becomes important for growth, arguing that Dam methylation exerts a critical function at the origin of replication itself. We propose that Dam methylation promotes DnaA-assisted successful duplex opening and replisome assembly at oriCI(Vc) in E. coli. In this model, methylation at oriCI(Vc) would ease DNA melting. This is supported by the fact that the requirement for dam can be alleviated by increasing negative supercoiling of the chromosome through oversupply of the DNA gyrase or loss of SeqA activity.
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