Evidence map›Paper›PMID 20511431›Full record

ArticleApplied and environmental microbiology2010

Unsuitability of quantitative Bacteroidales 16S rRNA gene assays for discerning fecal contamination of drinking water.

Paul W J J van der Wielen, Gertjan Medema

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
5.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 33 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Paul W J J van der WielenKWR Watercycle Research Institute, Nieuwegein, Netherlands. paul.van.der.wielen@kwrwater.nl
Gertjan Medema
KWR Water Research Institute · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacteroidales species were detected in (tap) water samples from treatment plants with three different PCR assays. 16S rRNA gene sequence analysis indicated that the sequences had an environmental rather than fecal origin. We conclude that assays for Bacteroidales 16S rRNA genes are not specific enough to discern fecal contamination of drinking water in the Netherlands.

Indexed as

Water MicrobiologyWater PollutionBacteroidetesCluster AnalysisColony Count, MicrobialDNA, BacterialDNA, RibosomalFecesMolecular Sequence DataNetherlandsPhylogenyPolymerase Chain ReactionRNA, Ribosomal, 16SSensitivity and SpecificitySequence Analysis, DNADNA, BacterialDNA, RibosomalRNA, Ribosomal, 16S

Identifiers

PMID20511431
PMCPMC2901713
OpenAlexW1977082928

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.