ArticleApplied and environmental microbiology2010
Engineering of Clostridium phytofermentans Endoglucanase Cel5A for improved thermostability.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 67 citations in OpenAlex.
- Biochemical Characterization of Phenylacetaldehyde Dehydrogenases from Styrene-degrading Soil Bacteria.Applied biochemistry and biotechnology · 2021Article
- Article
- Engineering Robust Cellulases for Tailored Lignocellulosic Degradation Cocktails.International journal of molecular sciences · 2020Review
- Directed Evolution ofInternational journal of molecular sciences · 2019Article
- Activity and Thermostability of GH5 Endoglucanase Chimeras from Mesophilic and Thermophilic Parents.Applied and environmental microbiology · 2019Article
- AnSynthetic and systems biotechnology · 2018Review
- Determination of glycoside hydrolase specificities during hydrolysis of plant cell walls using glycome profiling.Biotechnology for biofuels · 2017Article
- Global repositioning of transcription start sites in a plant-fermenting bacterium.Nature communications · 2016Article
- Polarity Alteration of a Calcium Site Induces a Hydrophobic Interaction Network and Enhances Cel9A Endoglucanase Thermostability.Applied and environmental microbiology · 2016Article
- Engineering Novel and Improved Biocatalysts by Cell Surface Display.Industrial & engineering chemistry research · 2015Article
- Mutagenesis of Trichoderma reesei endoglucanase I: impact of expression host on activity and stability at elevated temperatures.BMC biotechnology · 2015Article
- Multifunctional cellulase catalysis targeted by fusion to different carbohydrate-binding modules.Biotechnology for biofuels · 2015Article
- Site-Directed Mutagenesis of a Hyperthermophilic Endoglucanase Cel12B from Thermotoga maritima Based on Rational Design.PloS one · 2015Article
- Genome and Transcriptome of Clostridium phytofermentans, Catalyst for the Direct Conversion of Plant Feedstocks to Fuels.PloS one · 2015Article
- Insertion of endocellulase catalytic domains into thermostable consensus ankyrin scaffolds: effects on stability and cellulolytic activity.Applied and environmental microbiology · 2013Article
- Addition of a carbohydrate-binding module enhances cellulase penetration into cellulose substrates.Biotechnology for biofuels · 2013Article
- Polysaccharide hydrolysis with engineered Escherichia coli for the production of biocommodities.Journal of industrial microbiology & biotechnology · 2013Review
- Processivity and enzymatic mode of a glycoside hydrolase family 5 endoglucanase from Volvariella volvacea.Applied and environmental microbiology · 2013Article
- Improved activity of a thermophilic cellulase, Cel5A, from Thermotoga maritima on ionic liquid pretreated switchgrass.PloS one · 2013Article
- Engineering of family-5 glycoside hydrolase (Cel5A) from an uncultured bacterium for efficient hydrolysis of cellulosic substrates.PloS one · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A family 5 glycoside hydrolase from Clostridium phytofermentans was cloned and engineered through a cellulase cell surface display system in Escherichia coli. The presence of cell surface anchoring, a cellulose binding module, or a His tag greatly influenced the activities of wild-type and mutant enzymes on soluble and solid cellulosic substrates, suggesting the high complexity of cellulase engineering. The best mutant had 92%, 36%, and 46% longer half-lives at 60 degrees C on carboxymethyl cellulose, regenerated amorphous cellulose, and Avicel, respectively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.