ArticleThe Journal of antimicrobial chemotherapy2010
A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A.
Article in The Journal of antimicrobial chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- The TyphiNET data visualisation dashboard: unlocking Salmonella Typhi genomics data to support public health.Genome medicine · 2025Article
- Ecological prevalence, genetic diversity, and multidrug resistance of Salmonella enteritidis recovered from broiler and layer chicken farms.Poultry science · 2024Article
- Prevalence of Antimicrobial Resistance Genes inTropical medicine and infectious disease · 2022Review
- BlastFrost: fast querying of 100,000s of bacterial genomes in Bifrost graphs.Genome biology · 2021Article
- Carrion's Disease: the Sound of Silence.Clinical microbiology reviews · 2018Review
- Molecular Surveillance Identifies Multiple Transmissions of Typhoid in West Africa.PLoS neglected tropical diseases · 2016Article
- Subtyping of Salmonella enterica Subspecies I Using Single-Nucleotide Polymorphisms in Adenylate Cyclase.Foodborne pathogens and disease · 2016Article
- A Multicountry Molecular Analysis of Salmonella enterica Serovar Typhi With Reduced Susceptibility to Ciprofloxacin in Sub-Saharan Africa.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Article
- Simultaneous Detection of Major Drug Resistance Mutations of HIV-1 Subtype B Viruses from Dried Blood Spot Specimens by Multiplex Allele-Specific Assay.Journal of clinical microbiology · 2016Article
- Phylogeographical analysis of the dominant multidrug-resistant H58 clade of Salmonella Typhi identifies inter- and intracontinental transmission events.Nature genetics · 2015Article
- Genomic signature of multidrug-resistant Salmonella enterica serovar typhi isolates related to a massive outbreak in Zambia between 2010 and 2012.Journal of clinical microbiology · 2015Article
- Characterization of non-classical quinolone resistance inBiomolecular detection and quantification · 2014Article
- Lack of efflux mediated quinolone resistance in Salmonella enterica serovars Typhi and Paratyphi A.Frontiers in microbiology · 2014Article
- Emerging rapid resistance testing methods for clinical microbiology laboratories and their potential impact on patient management.BioMed research international · 2014Review
- Evolution of enterohemorrhagic escherichia coli O26 based on single-nucleotide polymorphisms.Genome biology and evolution · 2013Article
- "Spoligoriftyping," a dual-priming-oligonucleotide-based direct-hybridization assay for tuberculosis control with a multianalyte microbead-based hybridization system.Journal of clinical microbiology · 2012Article
- Molecular typing and resistance analysis of travel-associated Salmonella enterica serotype Typhi.Journal of clinical microbiology · 2012Article
- Transforming microbial genotyping: a robotic pipeline for genotyping bacterial strains.PloS one · 2012Article
- Salmonella enterica Serotype Typhi with nonclassical quinolone resistance phenotype.Emerging infectious diseases · 2011Article
- High-throughput bacterial SNP typing identifies distinct clusters of Salmonella Typhi causing typhoid in Nepalese children.BMC infectious diseases · 2010Article
Corrections and comments
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Authors and funding
8 authors at 6 institutions in 5 countries.
Funding
Abstract
objectivesDecreased susceptibility to fluoroquinolones has become a major problem for the successful therapy of human infections caused by Salmonella enterica, especially the life-threatening typhoid and paratyphoid fevers.
methodsBy using Luminex xTAG beads, we developed a rapid, reliable and cost-effective multiplexed genotyping assay for simultaneously detecting 11 mutations in gyrA, gyrB and parE of S. enterica serovars Typhi and Paratyphi A that result in nalidixic acid resistance (Nal(R)) and/or decreased susceptibility to fluoroquinolones.
resultsThis assay yielded unambiguous single nucleotide polymorphism calls on extracted DNA from 292 isolates of Salmonella Typhi (Nal(R) = 223 and Nal(S) = 69) and 106 isolates of Salmonella Paratyphi A (Nal(R) = 24 and Nal(S) = 82). All of the 247 Nal(R) Salmonella Typhi and Salmonella Paratyphi A isolates were found to harbour at least one of the target mutations, with GyrA Phe-83 as the most common one (143/223 for Salmonella Typhi and 18/24 for Salmonella Paratyphi A). We also identified three GyrB mutations in eight Nal(S) Salmonella Typhi isolates (six for GyrB Phe-464, one for GyrB Leu-465 and one for GyrB Asp-466), and mutations GyrB Phe-464 and GyrB Asp-466 seem to be related to the decreased ciprofloxacin susceptibility phenotype in Salmonella Typhi. This assay can also be used directly on boiled single colonies.
conclusionsThe assay presented here would be useful for clinical and reference laboratories to rapidly screen quinolone-resistant isolates of Salmonella Typhi and Salmonella Paratyphi A, and decipher the underlying genetic changes for epidemiological purposes.
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