ArticleThe Plant cell2010
Conserved alternative splicing of Arabidopsis transthyretin-like determines protein localization and S-allantoin synthesis in peroxisomes.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 80 citations in OpenAlex.
- Infection-responsive alternative splicing of GhZDS is associated with Verticillium wilt resistance in cotton.BMC plant biology · 2026Article
- A transthyretin-like protein acts downstream of miR397 and LACCASE to regulate grain yield in rice.The Plant cell · 2024Article
- Exogenous Allantoin Confers Rapeseed (Antioxidants (Basel, Switzerland) · 2023Article
- Catsnap: a user-friendly algorithm for determining the conservation of protein variants reveals extensive parallelisms in the evolution of alternative splicing.The New phytologist · 2023Article
- Identification of a key locus, qNL3.1, associated with seed germination under salt stress via a genome-wide association study in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Enzymes and cellular interplay required for flux of fixed nitrogen to ureides in bean nodules.Nature communications · 2022Article
- Alternative Splicing and Its Roles in Plant Metabolism.International journal of molecular sciences · 2022Review
- Inferred Subcellular Localization of Peroxisomal Matrix Proteins ofFrontiers in plant science · 2022Article
- How alternative splicing changes the properties of plant proteins.Quantitative plant biology · 2022Review
- Ureide metabolism in Arabidopsis thaliana is modulated by C:N balance.Journal of plant research · 2020Article
- TRANSTHYRETIN-LIKE and BYPASS1-LIKE co-regulate growth and cold tolerance in Arabidopsis.BMC plant biology · 2020Article
- Gene Duplication Accelerates the Pace of Protein Gain and Loss from Plant Organelles.Molecular biology and evolution · 2020Article
- Ureide Permease 5 (AtUPS5) Connects Cell Compartments Involved in Ureide Metabolism.Plant physiology · 2020Article
- Evolutionary Maintenance of the PTS2 Protein Import Pathway in the Stramenopile AlgaFrontiers in cell and developmental biology · 2020Article
- Nucleotide Metabolism in Plants.Plant physiology · 2020Article
- Allantoin reduces cell death induced by cisplatin: possible implications for tumor lysis syndrome management.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2019Article
- Opposite fates of the purine metabolite allantoin under water and nitrogen limitations in bread wheat.Plant molecular biology · 2019Article
- AMP and GMP Catabolism in Arabidopsis Converge on Xanthosine, Which Is Degraded by a Nucleoside Hydrolase Heterocomplex.The Plant cell · 2019Article
- Disruption of ureide degradation affects plant growth and development during and after transition from vegetative to reproductive stages.BMC plant biology · 2018Article
- Transcriptome Analysis of FourG3 (Bethesda, Md.) · 2018Article
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5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
S-allantoin, a major ureide compound, is produced in plant peroxisomes from oxidized purines. Sequence evidence suggested that the Transthyretin-like (TTL) protein, which interacts with brassinosteroid receptors, may act as a bifunctional enzyme in the synthesis of S-allantoin. Here, we show that recombinant TTL from Arabidopsis thaliana catalyzes two enzymatic reactions leading to the stereoselective formation of S-allantoin, hydrolysis of hydroxyisourate through a C-terminal Urah domain, and decarboxylation of 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline through an N-terminal Urad domain. We found that two different mRNAs are produced from the TTL gene through alternative use of two splice acceptor sites. The corresponding proteins differ in the presence (TTL(1-)) and the absence (TTL(2-)) of a rare internal peroxisomal targeting signal (PTS2). The two proteins have similar catalytic activity in vitro but different in vivo localization: TTL(1-) localizes in peroxisomes, whereas TTL(2-) localizes in the cytosol. Similar splice variants are present in monocots and dicots. TTL originated in green algae through a Urad-Urah fusion, which entrapped an N-terminal PTS2 between the two domains. The presence of this gene in all Viridiplantae indicates that S-allantoin biosynthesis has general significance in plant nitrogen metabolism, while conservation of alternative splicing suggests that this mechanism has general implications in the regulation of the ureide pathway in flowering plants.
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