ArticleThe Plant cell2010
Plastidial thioredoxin z interacts with two fructokinase-like proteins in a thiol-dependent manner: evidence for an essential role in chloroplast development in Arabidopsis and Nicotiana benthamiana.
Article in The Plant cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 149 papers.
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Who cites it
149 citing papers in PubMed, 328 citations in OpenAlex.
- A Chemical Probe for Increasing Leaf Tocopherol Levels by Coordinated Modulation of Biosynthesis, Competition and Storage.Plant biotechnology journal · 2026Article
- A red/blue optoswitch for temporal control of chloroplast transcription and biogenesis in Arabidopsis.Nature communications · 2026Article
- Map-based cloning identifies a missense SNP in CsTRX z, encoding a z-type thioredoxin homolog, as the genetic determinant of Dominant Virescent Leaf in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- A fungal pathogen suppresses host leaf senescence to increase infection.Nature communications · 2025Article
- OsPRDA1 Interacts With OsFSD2 To Promote Chloroplast Development by Regulating Chloroplast Gene Expression in Rice.Rice (New York, N.Y.) · 2025Article
- RAS, a Pentatricopeptide Repeat Protein, Interacts with OsTRX z to Regulate Chloroplast Gene Transcription and RNA Processing.Plants (Basel, Switzerland) · 2025Article
- Architecture of the spinach plastid-encoded RNA polymerase.Nature communications · 2024Article
- WHIRLY proteins, multi-layer regulators linking the nucleus and organelles in developmental and stress-induced senescence of plants.Annals of botany · 2024Review
- PAP1 and PAP7 are required for association of plastid-encoded RNA polymerase with DNA.Plant molecular biology · 2024Article
- The photoreactivation of 6 - 4 photoproducts in chloroplast and nuclear DNA depends on the amount of the Arabidopsis UV repair defective 3 protein.BMC plant biology · 2024Article
- The impact of light and thioredoxins on the plant thiol-disulfide proteome.Plant physiology · 2024Article
- Divergent Protein Redox Dynamics and Their Relationship with Electron Transport Efficiency during Photosynthesis Induction.Plant & cell physiology · 2024Article
- Cytological, Physiological, and Transcriptomic Analyses of the Leaf Color MutantPlants (Basel, Switzerland) · 2024Article
- Article
- PEP-ASSOCIATED PROTEIN 3 regulates rice tiller formation and grain yield by controlling chloroplast biogenesis.Plant physiology · 2024Article
- Modified Crosstalk between Phytohormones in Arabidopsis Mutants for PEP-Associated Proteins.International journal of molecular sciences · 2024Article
- WLP3 Encodes the Ribosomal Protein L18 and Regulates Chloroplast Development in Rice.Rice (New York, N.Y.) · 2023Article
- Redox regulation, thioredoxins, and glutaredoxins in retrograde signalling and gene transcription.Journal of experimental botany · 2023Review
- Chloroplast gene expression: Recent advances and perspectives.Plant communications · 2023Review
- Current Insights into the Redox Regulation Network in Plant Chloroplasts.Plant & cell physiology · 2023Review
89 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here, we characterize a plastidial thioredoxin (TRX) isoform from Arabidopsis thaliana that defines a previously unknown branch of plastidial TRXs lying between x- and y-type TRXs and thus was named TRX z. An Arabidopsis knockout mutant of TRX z had a severe albino phenotype and was inhibited in chloroplast development. Quantitative real-time RT-PCR analysis of the mutant suggested that the expressions of genes that depend on a plastid-encoded RNA polymerase (PEP) were specifically decreased. Similar results were obtained upon virus-induced gene silencing (VIGS) of the TRX z ortholog in Nicotiana benthamiana. We found that two fructokinase-like proteins (FLN1 and FLN2), members of the pfkB-carbohydrate kinase family, were potential TRX z target proteins and identified conserved Cys residues mediating the FLN-TRX z interaction. VIGS in N. benthamiana and inducible RNA interference in Arabidopsis of FLNs also led to a repression of PEP-dependent gene transcription. Remarkably, recombinant FLNs displayed no detectable sugar-phosphorylating activity, and amino acid substitutions within the predicted active site imply that the FLNs have acquired a new function, which might be regulatory rather than metabolic. We were able to show that the FLN2 redox state changes in vivo during light/dark transitions and that this change is mediated by TRX z. Taken together, our data strongly suggest an important role for TRX z and both FLNs in the regulation of PEP-dependent transcription in chloroplasts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.