ArticleThe Journal of biological chemistry2010
Yeast double-stranded RNA virus L-A deliberately synthesizes RNA transcripts with 5'-diphosphate.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Novel viruses of the family Partitiviridae discovered in Saccharomyces cerevisiae.PLoS pathogens · 2023Article
- Association of ScV-LA Virus with Host Protein Metabolism Determined by Proteomics Analysis and Cognate RNA Sequencing.Viruses · 2022Article
- Structures of L-BC virus and its open particle provide insight into Totivirus capsid assembly.Communications biology · 2022Article
- Adaptive Response ofJournal of fungi (Basel, Switzerland) · 2022Article
- A Simple Multiplex Reverse Transcription-PCR Method for the Diagnosis of L-A and M Totiviruses in Saccharomyces cerevisiae.Applied and environmental microbiology · 2022Article
- Structure and Sequence Requirements for RNA Capping at the Venezuelan Equine Encephalitis Virus RNA 5' End.Journal of virology · 2021Article
- A recap of RNA recapping.Wiley interdisciplinary reviews. RNA · 2019Review
- XRN1 Is a Species-Specific Virus Restriction Factor in Yeasts.PLoS pathogens · 2016Article
- Article
- Cap snatching in yeast L-BC double-stranded RNA totivirus.The Journal of biological chemistry · 2013Article
- Viruses and prions of Saccharomyces cerevisiae.Advances in virus research · 2013Review
- Cap snatching of yeast L-A double-stranded RNA virus can operate in trans and requires viral polymerase actively engaging in transcription.The Journal of biological chemistry · 2012Article
- Cap-snatching mechanism in yeast L-A double-stranded RNA virus.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Strategies for viral RNA stability: live long and prosper.Trends in genetics : TIG · 2011Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
L-A is a persistent double-stranded RNA virus commonly found in the yeast Saccharomyces cerevisiae. Isolated L-A virus synthesizes positive strand transcripts in vitro. We found that the 5' termini of the transcripts are diphosphorylated. The 5'-terminal nucleotide is G, and GDP was the best substrate among those examined to prime the reaction. When GTP was used, the triphosphate of GTP incorporated into the 5'-end was converted to diphosphate. This activity was not dependent on host CTL1 RNA triphosphatase. The 5'-end of the GMP-primed transcript also was converted to diphosphate, the beta-phosphate of which was derived from the gamma-phosphate of ATP present in the polymerization reaction. These results demonstrate that L-A virus commands elaborate enzymatic systems to ensure its transcript to be 5'-diphosphorylated. Transcripts of M1, a satellite RNA of L-A virus, also had diphosphate at the 5' termini. Because viral transcripts are released from the virion into the cytoplasm to be translated and encapsidated into a new viral particle, a stage most vulnerable to degradation in the virus replication cycle, our results suggest that the 5'-diphosphate status is important for transcript stability. Consistent with this, L-A transcripts made in vitro are resistant to the affinity-purified Ski1p 5'-exonuclease. We also discuss the implication of these findings on translation of viral RNA. Because the viral transcript has no conventional 5'-cap structure, this work may shed light on the metabolism of non-self-RNA in yeast.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.