ArticleBiomaterials2010
The dependence of MG63 osteoblast responses to (meth)acrylate-based networks on chemical structure and stiffness.
Article in Biomaterials, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Spatiotemporally Controlled Photoresponsive Hydrogels: Design and Predictive Modeling from Processing through Application.Advanced functional materials · 2020Review
- Decoupling the effects of nanopore size and surface roughness on the attachment, spreading and differentiation of bone marrow-derived stem cells.Biomaterials · 2020Article
- A Patterned Butyl Methacrylate-Polymers · 2019Article
- Phage-Based Artificial Niche: The Recent Progress and Future Opportunities in Stem Cell Therapy.Stem cells international · 2019Review
- Osteogenesis-Related Behavior of MC3T3-E1 Cells on Substrates with Tunable Stiffness.BioMed research international · 2018Article
- Fabrication of Trabecular Bone-Templated Tissue-Engineered Constructs by 3D Inkjet Printing.Advanced healthcare materials · 2017Article
- Substrate Stiffness Controls Osteoblastic and Chondrocytic Differentiation of Mesenchymal Stem Cells without Exogenous Stimuli.PloS one · 2017Article
- 3D Printing of Tissue Engineered Constructs for In Vitro Modeling of Disease Progression and Drug Screening.Annals of biomedical engineering · 2017Review
- Substrate Modulus Regulates Osteogenic Differentiation of Rat Mesenchymal Stem Cells through Integrin β1 and BMP Receptor Type IA.Journal of materials chemistry. B · 2016Article
- Fabrication of 3D Scaffolds with Precisely Controlled Substrate Modulus and Pore Size by Templated-Fused Deposition Modeling to Direct Osteogenic Differentiation.Advanced healthcare materials · 2015Article
- Article
- Surface Properties of Nanostructured Bio-Active Interfaces: Impacts of Surface Stiffness and Topography on Cell-Surface Interactions.RSC advances · 2013Article
- Degradable segmented polyurethane elastomers for bone tissue engineering: effect of polycaprolactone content.Journal of biomaterials science. Polymer edition · 2013Article
- Osteoblasts exhibit a more differentiated phenotype and increased bone morphogenetic protein production on titanium alloy substrates than on poly-ether-ether-ketone.The spine journal : official journal of the North American Spine Society · 2012Article
- The influence of elasticity and surface roughness on myogenic and osteogenic-differentiation of cells on silk-elastin biomaterials.Biomaterials · 2011Article
- Contribution of bone tissue modulus to breast cancer metastasis to bone.Cancer microenvironment : official journal of the International Cancer Microenvironment Society · 2011Article
- Marrow-derived stem cell motility in 3D synthetic scaffold is governed by geometry along with adhesivity and stiffness.Biotechnology and bioengineering · 2011Article
- Sensing and modulation of invadopodia across a wide range of rigidities.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The cell response to an implant is regulated by the implant's surface properties including topography and chemistry, but less is known about how the mechanical properties affect cell behavior. The objective of this study was to evaluate how the surface stiffness and chemistry of acrylate-based copolymer networks affect the in vitro response of human MG63 pre-osteoblast cells. Networks comprised of poly(ethylene glycol) dimethacrylate (PEGDMA; Mn approximately 750) and diethylene glycol dimethacrylate (DEGDMA) were photopolymerized at different concentrations to produce three compositions with moduli ranging from 850 to 60 MPa. To further decouple chemistry and stiffness, three networks comprised of 2-hydroxyethyl methacrylate (2HEMA) and PEGDMA or DEGDMA were also designed that exhibited a range of moduli similar to the PEGDMA-DEGDMA networks. MG63 cells were cultured on each surface and tissue culture polystyrene (TCPS), and the effect of copolymer composition on cell number, osteogenic markers (alkaline phosphatase specific activity and osteocalcin), and local growth factor production (OPG, TGF-beta1, and VEGF-A) were assessed. Cells exhibited a more differentiated phenotype on the PEGDMA-DEGDMA copolymers compared to the 2HEMA-PEGDMA copolymers. On the PEGDMA-DEGDMA system, cells exhibited a more differentiated phenotype on the stiffest surface indicated by elevated osteocalcin compared with TCPS. Conversely, cells on 2HEMA-PEGDMA copolymers became more differentiated on the less stiff 2HEMA surface. Growth factors were regulated in a differential manner. These results indicate that copolymer chemistry is the primary regulator of osteoblast differentiation, and the effect of stiffness is secondary to the surface chemistry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.