ArticleMicrobial pathogenesis2010
Autoprocessing and self-activation of the secreted protease CPAF in Chlamydia-infected cells.
Article in Microbial pathogenesis, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 25 citations in OpenAlex.
- Chlamydia trachomatis paralyses neutrophils to evade the host innate immune response.Nature microbiology · 2018Article
- Peptidase Inhibitor 15 (PI15) Regulates Chlamydial CPAF Activity.Frontiers in cellular and infection microbiology · 2018Article
- Chlamydial Plasmid-Dependent Pathogenicity.Trends in microbiology · 2017Review
- Article
- The Chlamydia-Secreted Protease CPAF Promotes Chlamydial Survival in the Mouse Lower Genital Tract.Infection and immunity · 2016Article
- Characterization of CPAF critical residues and secretion during Chlamydia trachomatis infection.Infection and immunity · 2015Article
- The Chlamydia protease CPAF: caution, precautions and function.Pathogens and disease · 2014Article
- Analysis of CPAF mutants: new functions, new questions (the ins and outs of a chlamydial protease).Pathogens and disease · 2014Article
- A path forward for the chlamydial virulence factor CPAF.Microbes and infection · 2013Review
- Lost after translation: post-translational modifications by bacterial type III effectors.Current opinion in microbiology · 2013Review
- Chlamydia trachomatis outer membrane complex protein B (OmcB) is processed by the protease CPAF.Journal of bacteriology · 2013Article
- Distinct intensity of host-pathogen interactions in Chlamydia psittaci- and Chlamydia abortus-infected chicken embryos.Infection and immunity · 2012Article
- Cleavage of a putative metal permease in Chlamydia trachomatis yields an iron-dependent transcriptional repressor.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Persistent Chlamydia trachomatis infection of HeLa cells mediates apoptosis resistance through a Chlamydia protease-like activity factor-independent mechanism and induces high mobility group box 1 release.Infection and immunity · 2012Article
- CPAF: a Chlamydial protease in search of an authentic substrate.PLoS pathogens · 2012Article
- The chlamydial periplasmic stress response serine protease cHtrA is secreted into host cell cytosol.BMC microbiology · 2011Article
- Chlamydia trachomatis secretion of proteases for manipulating host signaling pathways.Frontiers in microbiology · 2011Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
Abstract
The Chlamydia-secreted protease/proteasome-like activity factor (CPAF) is synthesized as a proenzyme (proCPAF) and requires processing for proteolytic activity. Recent structural studies have further demonstrated that CPAF is a serine protease that can undergo autoprocessing and self-activation in a concentration-dependent manner in vitro. However, it is not known how CPAF is processed and activated during chlamydial infection. In the current study, we used a mutant CPAF designated as CPAF(E558A) that is deficient in processing by itself as a substrate to search for putative CPAF activation factor(s) in Chlamydia-infected cells. CPAF(E558A) was processed by the lysates made from Chlamydia-infected cells and the processing activity correlated with the presence of endogenous active CPAF in the fractionated lysate samples. CPAF produced in the Chlamydia-infected cells is required for processing the mutant CPAF(E558A) since the processing activity was removed by depletion with anti-CPAF but not control antibodies. Furthermore, a purified and activated wild type CPAF alone was sufficient for processing CPAF(E558A) and no other chlamydial proteases are required. Finally, fusion tag-induced oligomerization can lead to autoprocessing and self-activation of the wild type CPAF in mammalian cells. These observations together have demonstrated that CPAF undergoes autoprocessing and self-activation during chlamydial infection.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.