ArticleBMC genomics2010
Transcriptome analysis in non-model species: a new method for the analysis of heterologous hybridization on microarrays.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Human Respiratory Syncytial Virus-Induced Immune Signature of Infection Revealed by Transcriptome Analysis of Clinical Pediatric Nasopharyngeal Swab Samples.The Journal of infectious diseases · 2021Article
- Molecular Effects of Silver Nanoparticles on Monogenean Parasites: Lessons fromInternational journal of molecular sciences · 2020Article
- Repurposing of Drugs as Novel Influenza Inhibitors From Clinical Gene Expression Infection Signatures.Frontiers in immunology · 2019Article
- Comparative transcriptomic analysis of the evolution and development of flower size in Saltugilia (Polemoniaceae).BMC genomics · 2017Article
- Transcriptomic data analysis and differential gene expression of antioxidant pathways in king penguin juveniles (Data in brief · 2016Article
- New Approaches to Comparative and Animal Stress Biology Research in the Post-genomic Era: A Contextual Overview.Computational and structural biotechnology journal · 2014Review
- Evolutionary insights into scleractinian corals using comparative genomic hybridizations.BMC genomics · 2012Article
- Selective upregulation of lipid metabolism in skeletal muscle of foraging juvenile king penguins: an integrative study.Proceedings. Biological sciences · 2012Article
- Plasticity of the myelination genomic fabric.Molecular genetics and genomics : MGG · 2012Article
- Normalization and centering of array-based heterologous genome hybridization based on divergent control probes.BMC bioinformatics · 2011Article
- In vitro homology search array comprehensively reveals highly conserved genes and their functional characteristics in non-sequenced species.BMC genomics · 2010Article
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9 authors.
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Abstract
backgroundRecent developments in high-throughput methods of analyzing transcriptomic profiles are promising for many areas of biology, including ecophysiology. However, although commercial microarrays are available for most common laboratory models, transcriptome analysis in non-traditional model species still remains a challenge. Indeed, the signal resulting from heterologous hybridization is low and difficult to interpret because of the weak complementarity between probe and target sequences, especially when no microarray dedicated to a genetically close species is available.
resultsWe show here that transcriptome analysis in a species genetically distant from laboratory models is made possible by using MAXRS, a new method of analyzing heterologous hybridization on microarrays. This method takes advantage of the design of several commercial microarrays, with different probes targeting the same transcript. To illustrate and test this method, we analyzed the transcriptome of king penguin pectoralis muscle hybridized to Affymetrix chicken microarrays, two organisms separated by an evolutionary distance of approximately 100 million years. The differential gene expression observed between different physiological situations computed by MAXRS was confirmed by real-time PCR on 10 genes out of 11 tested.
conclusionsMAXRS appears to be an appropriate method for gene expression analysis under heterologous hybridization conditions.
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