ArticleBMC microbiology2010
Natural transformation of Vibrio cholerae as a tool--optimizing the procedure.
Article in BMC microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 93 citations in OpenAlex.
- Evolutionary constraints on RNA polymerase gene positioning in the genome of fast-growing bacteria.Nucleic acids research · 2026Article
- Bacterial natural transformation drives cassette shuffling and simplifies recombination in chromosomal integrons.Nucleic acids research · 2025Article
- Investigating the molecular transmission dynamics ofJournal of bacteriology · 2025Article
- The carRS-ompV-virK operon of Vibrio cholerae senses antimicrobial peptides and activates the expression of multiple resistance systems.Scientific reports · 2025Article
- Deployment of amBio · 2025Article
- Chromosomal integrons are genetically and functionally isolated units of genomes.Nucleic acids research · 2024Article
- Fine-tuning of a CRISPRi screen in the seventh pandemic Vibrio cholerae.BMC genomics · 2024Article
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- Deployment of abioRxiv : the preprint server for biology · 2024Article
- Sporadic type VI secretion in seventh pandemicMicrobiology (Reading, England) · 2023Article
- Protozoal food vacuoles enhance transformation in Vibrio cholerae through SOS-regulated DNA integration.The ISME journal · 2022Article
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- The VarA-CsrA regulatory pathway influences cell shape in Vibrio cholerae.PLoS genetics · 2022Article
- A Strategy for the Rapid Development of a SafeInternational journal of molecular sciences · 2021Article
- A New Contact Killing Toxin Permeabilizes Cells and Belongs to a Broadly Distributed Protein Family.mSphere · 2021Article
- Cassette recruitment in the chromosomal Integron of Vibrio cholerae.Nucleic acids research · 2021Article
- Natural Transformation in a Classical-Biotype Vibrio cholerae Strain.Applied and environmental microbiology · 2021Article
- Interbacterial competition and anti-predatory behaviour of environmental Vibrio cholerae strains.Environmental microbiology · 2020Article
- Comparison of chitin-induced natural transformation in pandemic Vibrio cholerae O1 El Tor strains.Environmental microbiology · 2020Article
- Regulation of Chitin-Dependent Growth and Natural Competence inMicroorganisms · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundVibrio cholerae gains natural competence upon growth on chitin. This allows the organism to take up free DNA from the environment and to incorporate it into its genome by homologous recombination.
resultsMaking use of this developmental program in order to use it as a tool to genetically manipulate V. cholerae and potentially also others Vibrio species was envisaged. Therefore, we re-investigated the experimental design for natural transformation of V. cholerae and tested different donor DNA fragments with respect to their source (genomic versus PCR-derived), quantity, and homologous flanking regions. Furthermore, we simplified the procedure in terms of the chitin source used as inducer of natural competence and the composition of the growth medium.
conclusionsThe current study allows us to recommend a standard protocol to genetically manipulate V. cholerae using commercially available sources of chitin and minimal medium, respectively, as well as PCR-derived donor DNA as transforming material.
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