ArticleJournal of plant research2011
RNAi-mediated suppression of the phenylalanine ammonia-lyase gene in Salvia miltiorrhiza causes abnormal phenotypes and a reduction in rosmarinic acid biosynthesis.
Article in Journal of plant research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39 citing papers in PubMed, 135 citations in OpenAlex.
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- Citrulline enhances salinity tolerance via photosynthesis, redox balance, osmotic and hormonal regulation, and nutrient assimilation in sunflower (Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Enhancement of Rosmarinic Acid Production in Hairy Root Cultures ofInternational journal of molecular sciences · 2025Article
- Comparative analysis of the PAL gene family in nine citruses provides new insights into the stress resistance mechanism of Citrus species.BMC genomics · 2024Article
- Monitoring of parasite Orobanche cumana using Vis-NIR hyperspectral imaging combining with physio-biochemical parameters on host crop Helianthus annuus.Plant cell reports · 2024Article
- Systematic characterization of gene families and functional analysis ofFrontiers in plant science · 2024Article
- A tracking work on how Sm4CL2 re-directed the biosynthesis of salvianolic acids and tanshinones in Salvia miltiorrhiza hairy roots.Plant cell reports · 2023Article
- Genome-wide family prediction unveils molecular mechanisms underlying the regulation of agronomic traits inFrontiers in plant science · 2023Article
- Integrated metabolomic and transcriptomic analyses of regulatory mechanisms associated with uniconazole-induced dwarfism in banana.BMC plant biology · 2022Article
- A Protective Role of Phenylalanine Ammonia-Lyase fromInternational journal of molecular sciences · 2022Article
- SmbHLH60 and SmMYC2 antagonistically regulate phenolic acids and anthocyanins biosynthesis in Salvia miltiorrhiza.Journal of advanced research · 2022Article
- Ocimum sanctum, OscWRKY1, regulates phenylpropanoid pathway genes and promotes resistance to pathogen infection in Arabidopsis.Plant molecular biology · 2022Article
- Article
- Relationship between the Phenylpropanoid Pathway and Dwarfism ofInternational journal of molecular sciences · 2021Article
- Enhancing the expression ofRoyal Society open science · 2020Article
- Review
- Characterization of promoter of EgPAL1, a novel PAL gene from the oil palm Elaeis guineensis Jacq.Plant cell reports · 2018Article
- SmbHLH37 Functions Antagonistically With SmMYC2 in Regulating Jasmonate-Mediated Biosynthesis of Phenolic Acids inFrontiers in plant science · 2018Article
- Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Medicinal Salvia miltiorrhiza contains two main groups of active pharmaceutical ingredients: lipid-soluble tanshinones and water-soluble phenolic acids, including rosmarinic acid and salvianolic acid B. Phenylalanine ammonia-lyase (PAL) catalyzes the first step in the phenylpropanoid pathway and is assumed to be closely related to the accumulation of rosmarinic acid and its derivatives. We selected a 217-bp fragment, located at the 3' end of the coding region of PAL1, to establish an RNA interference construct that was introduced into S. miltiorrhiza via Agrobacterium tumefaciens-mediated transformation. PAL-suppressed plants exhibited several unusual phenotypes such as stunted growth, delayed root formation, altered leaves, and reduced lignin deposition. The total phenolic content was decreased by 20-70% in PAL-suppressed lines, and was accompanied by lower PAL activity. Down-regulation of PAL also affected the expression of C4H, 4CL2, and TAT, which are related genes in the rosmarinic acid pathway. Moreover, rosmarinic acid and salvianolic acid B were markedly reduced in PAL-suppressed lines, as detected by HPLC analysis. Our results indicate that PAL is very important for the synthesis of major water-soluble pharmaceutical ingredients within S. miltiorrhiza.
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