ArticleGene therapy2010
Ultrasound-assisted non-viral gene transfer to the salivary glands.
Article in Gene therapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Advances in gene transfer technologies: comparing viral and non-viral vectors for therapeutic applications.3 Biotech · 2026Review
- Advances in Gene Therapy for Xerostomia: Current Perspectives and Future Directions: A Narrative Review.Health science reports · 2026Article
- Intraoperative Visualization and Treatment of Salivary Gland Dysfunction in Sjögren's Syndrome Patients Using Contrast-Enhanced Ultrasound Sialendoscopy (CEUSS).Journal of clinical medicine · 2023Article
- Gene Therapeutic Delivery to the Salivary Glands.Advances in experimental medicine and biology · 2023Article
- In Vivo Transfection of Rat Salivary Glands With Fluorescently Tagged Aquaporin-5 Channel DNA.Cureus · 2022Article
- Diagnosis, Prevention, and Treatment of Radiotherapy-Induced Xerostomia: A Review.Journal of oncology · 2022Review
- Recent Advances in Gene Therapy for Cancer Theranostics.Current opinion in biomedical engineering · 2021Article
- Tetravalent dengue DNA vaccine is not immunogenic when delivered by retrograde infusion into salivary glands.Tropical diseases, travel medicine and vaccines · 2020Article
- In vivo Endocrine Secretion of Prostacyclin Following Expression of a Cyclooxygenase-1/Prostacyclin Fusion Protein in the Salivary Glands of Rats Via Nonviral Gene Therapy.Human gene therapy · 2017Article
- Ultrasound-targeted microbubble destruction in gene therapy: A new tool to cure human diseases.Genes & diseases · 2017Review
- Promising Gene Therapeutics for Salivary Gland Radiotoxicity.AIMS medical science · 2016Article
- Ultrasound-assisted nonviral gene transfer of AQP1 to the irradiated minipig parotid gland restores fluid secretion.Gene therapy · 2015Article
- IL-17 sequestration via salivary gland gene therapy in a mouse model of Sjogren's syndrome suppresses disease-associated expression of the putative autoantigen Klk1b22.Arthritis research & therapy · 2015Article
- Advances in salivary gland gene therapy - oral and systemic implications.Expert opinion on biological therapy · 2015Review
- Proteomic profiling of salivary gland after nonviral gene transfer mediated by conventional plasmids and minicircles.Molecular therapy. Methods & clinical development · 2014Article
- Cost-effectiveness landscape analysis of treatments addressing xerostomia in patients receiving head and neck radiation therapy.Oral surgery, oral medicine, oral pathology and oral radiology · 2013Review
- In vitro gene delivery with ultrasound-triggered polymer microbubbles.Ultrasound in medicine & biology · 2013Article
- Plasmid DNA is internalized from the apical plasma membrane of the salivary gland epithelium in live animals.Histochemistry and cell biology · 2012Article
- Sonoporation delivery of interleukin-27 gene therapy efficiently reduces prostate tumor cell growth in vivo.Human gene therapy · 2011Article
- Gene delivery in salivary glands: from the bench to the clinic.Biochimica et biophysica acta · 2011Review
Corrections and comments
- Erratum issuedDosage error in article text
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
We report a non-viral gene transfer method using ultrasound induced microbubble destruction to allow the uptake of plasmid gene transfer vectors to the cells of the mouse salivary gland. The Luciferase (Luc) reporter gene, driven by a cytomegalovirus (CMV) promoter, was delivered unilaterally to the submandibular salivary gland via retroductal cannulation and Luc expression was monitored with in vivo imaging. The CMV-Luc plasmid was delivered to the salivary gland in a carrier solution containing microbubbles composed of lipid-encased perfluoropropane gas, with two different concentrations of microbubbles used (100 and 15% volume/volume). An Adenoviral (Ad) vector using an identical CMV-Luc expression cassette was used as a positive control at two different dosages. Whereas ultrasound-assisted gene transfer (UAGT) with 100% microbubbles was weak and rapidly extinguished, UAGT with the 15% microbubble solution was robust and stable for 28 days. UAGT seems to be a practicable and promising method for non-viral gene delivery to the salivary glands.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.