ArticleProceedings of the National Academy of Sciences of the United States of America2010
Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 94 papers.
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Who cites it
94 citing papers in PubMed, 306 citations in OpenAlex.
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- CRISPR-based gene editing in plants: Focus on reagents and their delivery tools.BioImpacts : BI · 2025Review
- Recent advances in site-specific transgene insertion into the maize genome using recombinases and genome editing endonucleases.Frontiers in plant science · 2025Review
- Improvement of genome editing efficiency by Cas9 codon optimization in Japanese cedar (Plant biotechnology (Tokyo, Japan) · 2024Article
- Genomic Survey of LRR-RLK Genes inPlants (Basel, Switzerland) · 2024Article
- Characterization, evolution, and abiotic stress responses of leucine-rich repeat receptor-like protein kinases (LRR-RLK) in Liriodendron chinense.BMC genomics · 2024Article
- CRISPR/Cas9-mediated genome editing technique to control fall armyworm (Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Review
- Recent Advances in Tomato Gene Editing.International journal of molecular sciences · 2024Review
- Advancements in genome editing tools for genetic studies and crop improvement.Frontiers in plant science · 2024Review
- Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production.Frontiers in genome editing · 2024Review
- Tools and targets: The dual role of plant viruses in CRISPR-Cas genome editing.The plant genome · 2023Review
- Comparative analysis of seed and seedling irradiation with gamma rays and carbon ions for mutation induction in Arabidopsis.Frontiers in plant science · 2023Article
- Genome editing technologies, mechanisms and improved production of therapeutic phytochemicals: Opportunities and prospects.Biotechnology and bioengineering · 2023Review
- CRISPR/Cas technology for improving nutritional values in the agricultural sector: an update.Molecular biology reports · 2022Review
- Review
- Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants.Frontiers in genome editing · 2022Review
- Development of the binary vector pTACAtg1 for stable gene expression in plant: Reduction of gene silencing in transgenic plants carrying the target gene with long flanking sequences.Plant biotechnology (Tokyo, Japan) · 2021Article
- Hairy CRISPR: Genome Editing in Plants Using Hairy Root Transformation.Plants (Basel, Switzerland) · 2021Review
34 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Site-directed mutagenesis in higher plants remains a significant technical challenge for basic research and molecular breeding. Here, we demonstrate targeted-gene inactivation for an endogenous gene in Arabidopsis using zinc finger nucleases (ZFNs). Engineered ZFNs for a stress-response regulator, the ABA-INSENSITIVE4 (ABI4) gene, cleaved their recognition sequences specifically in vitro, and ZFN genes driven by a heat-shock promoter were introduced into the Arabidopsis genome. After heat-shock induction, gene mutations with deletion and substitution in the ABI4 gene generated via ZFN-mediated cleavage were observed in somatic cells at frequencies as high as 3%. The homozygote mutant line zfn_abi4-1-1 for ABI4 exhibited the expected mutant phenotypes, i.e., ABA and glucose insensitivity. In addition, ZFN-mediated mutagenesis was applied to the DNA repair-deficient mutant plant, atku80. We found that lack of AtKu80, which plays a role in end-protection of dsDNA breaks, increased error-prone rejoining frequency by 2.6-fold, with increased end-degradation. These data demonstrate that an approach using ZFNs can be used for the efficient production of mutant plants for precision reverse genetics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.