ArticleNucleic acids research2010
Ratiometric bimolecular beacons for the sensitive detection of RNA in single living cells.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 58 citations in OpenAlex.
- DNA/RNA Fluorescence Imaging by Synthetic Nucleic Acids.Advances in experimental medicine and biology · 2021Article
- Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging.iScience · 2020Review
- Imaging of DNA and RNA in Living Eukaryotic Cells to Reveal Spatiotemporal Dynamics of Gene Expression.Annual review of biochemistry · 2020Review
- Single-molecule analysis of endogenous β-actin mRNA trafficking reveals a mechanism for compartmentalized mRNA localization in axons.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Single-mRNA detection in living S. cerevisiae using a re-engineered MS2 system.Nature protocols · 2018Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- A CRISPR/molecular beacon hybrid system for live-cell genomic imaging.Nucleic acids research · 2018Article
- Engineering Novel Molecular Beacon Constructs to Study Intracellular RNA Dynamics and Localization.Genomics, proteomics & bioinformatics · 2017Review
- A molecular beacon-based approach for live-cell imaging of RNA transcripts with minimal target engineering at the single-molecule level.Scientific reports · 2017Article
- Non-genetic Purification of Ventricular Cardiomyocytes from Differentiating Embryonic Stem Cells through Molecular Beacons Targeting IRX-4.Stem cell reports · 2015Article
- DNA nanotechnology from the test tube to the cell.Nature nanotechnology · 2015Review
- Single-cell detection of mRNA expression using nanofountain-probe electroporated molecular beacons.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Real-time imaging of single engineered RNA transcripts in living cells using ratiometric bimolecular beacons.Journal of visualized experiments : JoVE · 2014Article
- A color-tunable molecular beacon to sense miRNA-9 expression during neurogenesis.Scientific reports · 2014Article
- Article
- Imaging the directed transport of single engineered RNA transcripts in real-time using ratiometric bimolecular beacons.PloS one · 2014Article
- Fluorescent probes for nucleic Acid visualization in fixed and live cells.Molecules (Basel, Switzerland) · 2013Review
- Quantitative assessment of ratiometric bimolecular beacons as a tool for imaging single engineered RNA transcripts and measuring gene expression in living cells.Nucleic acids research · 2013Article
- Rapid oligonucleotide-templated fluorogenic tetrazine ligations.Nucleic acids research · 2013Article
- Imaging mRNA expression levels in living cells with PNA·DNA binary FRET probes delivered by cationic shell-crosslinked nanoparticles.Organic & biomolecular chemistry · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Numerous studies have utilized molecular beacons (MBs) to image RNA expression in living cells; however, there is growing evidence that the sensitivity of RNA detection is significantly hampered by their propensity to emit false-positive signals. To overcome these limitations, we have developed a new RNA imaging probe called ratiometric bimolecular beacon (RBMB), which combines functional elements of both conventional MBs and siRNA. Analogous to MBs, RBMBs elicit a fluorescent reporter signal upon hybridization to complementary RNA. In addition, an siRNA-like double-stranded domain is used to facilitate nuclear export. Accordingly, live-cell fluorescent imaging showed that RBMBs are localized predominantly in the cytoplasm, whereas MBs are sequestered into the nucleus. The retention of RBMBs within the cytoplasmic compartment led to >15-fold reduction in false-positive signals and a significantly higher signal-to-background compared with MBs. The RBMBs were also designed to possess an optically distinct reference fluorophore that remains unquenched regardless of probe confirmation. This reference dye not only provided a means to track RBMB localization, but also allowed single cell measurements of RBMB fluorescence to be corrected for variations in probe delivery. Combined, these attributes enabled RBMBs to exhibit an improved sensitivity for RNA detection in living cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.