ArticleMicrobial cell factories2010
PGM2 overexpression improves anaerobic galactose fermentation in Saccharomyces cerevisiae.
Article in Microbial cell factories, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 42 citations in OpenAlex.
- TRAF3: A novel regulator of mitochondrial physiology and metabolic pathways in B lymphocytes.Frontiers in oncology · 2023Review
- Past, Present, and Future Perspectives on Whey as a Promising Feedstock for Bioethanol Production by Yeast.Journal of fungi (Basel, Switzerland) · 2022Review
- Reconstituting the complete biosynthesis of D-lysergic acid in yeast.Nature communications · 2022Article
- D-Xylose Sensing inInternational journal of molecular sciences · 2021Review
- Advances in biotechnological production of santalenes and santalols.Chinese herbal medicines · 2021Review
- MAL62 overexpression enhances uridine diphosphoglucose-dependent trehalose synthesis and glycerol metabolism for cryoprotection of baker's yeast in lean dough.Microbial cell factories · 2020Article
- Repeated Cis-Regulatory Tuning of a Metabolic Bottleneck Gene during Evolution.Molecular biology and evolution · 2018Article
- A Mutation inApplied and environmental microbiology · 2018Article
- Comparative and network-based proteomic analysis of low dose ethanol- and lipopolysaccharide-induced macrophages.PloS one · 2018Article
- Ongoing resolution of duplicate gene functions shapes the diversification of a metabolic network.eLife · 2016Article
- Enhanced leavening ability of baker's yeast by overexpression of SNR84 with PGM2 deletion.Journal of industrial microbiology & biotechnology · 2015Article
- Carbon source dependent promoters in yeasts.Microbial cell factories · 2014Review
- Carbon dioxide fixation by Calvin-Cycle enzymes improves ethanol yield in yeast.Biotechnology for biofuels · 2013Article
- Recovery of phenotypes obtained by adaptive evolution through inverse metabolic engineering.Applied and environmental microbiology · 2012Article
- Optimality principles in the regulation of metabolic networks.Metabolites · 2012Article
- Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries.Cellular and molecular life sciences : CMLS · 2012Review
- Cross-reactions between engineered xylose and galactose pathways in recombinant Saccharomyces cerevisiae.Biotechnology for biofuels · 2010Article
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3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundIn Saccharomyces cerevisiae galactose is initially metabolized through the Leloir pathway after which glucose 6-phosphate enters glycolysis. Galactose is controlled both by glucose repression and by galactose induction. The gene PGM2 encodes the last enzyme of the Leloir pathway, phosphoglucomutase 2 (Pgm2p), which catalyses the reversible conversion of glucose 1-phosphate to glucose 6-phosphate. Overexpression of PGM2 has previously been shown to enhance aerobic growth of S. cerevisiae in galactose medium.
resultsIn the present study we show that overexpression of PGM2 under control of the HXT7'promoter from an integrative plasmid increased the PGM activity 5 to 6 times, which significantly reduced the lag phase of glucose-pregrown cells in an anaerobic galactose culture. PGM2 overexpression also increased the anaerobic specific growth rate whereas ethanol production was less influenced. When PGM2 was overexpressed from a multicopy plasmid instead, the PGM activity increased almost 32 times. However, this increase of PGM activity did not further improve aerobic galactose fermentation as compared to the strain carrying PGM2 on the integrative plasmid.
conclusionPGM2 overexpression in S. cerevisiae from an integrative plasmid is sufficient to reduce the lag phase and to enhance the growth rate in anaerobic galactose fermentation, which results in an overall decrease in fermentation duration. This observation is of particular importance for the future development of stable industrial strains with enhanced PGM activity.
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