ArticleGenesis (New York, N.Y. : 2000)2010
Characterization of bacterial artificial chromosome transgenic mice expressing mCherry fluorescent protein substituted for the murine smooth muscle alpha-actin gene.
Article in Genesis (New York, N.Y. : 2000), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 29 citations in OpenAlex.
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- Single Cell Sequencing Reveals Mechanisms of Persistent Truncus Arteriosus Formation afterGenes · 2022Article
- Contractile apparatus in CNS capillary pericytes.Neurophotonics · 2022Review
- Imaging and optogenetic modulation of vascular mural cells in the live brain.Nature protocols · 2021Article
- Nkx2-5 defines distinct scaffold and recruitment phases during formation of the murine cardiac Purkinje fiber network.Nature communications · 2020Article
- Neuronal regulation of the blood-brain barrier and neurovascular coupling.Nature reviews. Neuroscience · 2020Review
- Second harmonic generation microscopy of early embryonic mouse hearts.Biomedical optics express · 2019Article
- Nanofiber/Microsphere Hybrid MatricesRegenerative engineering and translational medicine · 2018Article
- Overview of the reporter genes and reporter mouse models.Animal models and experimental medicine · 2018Review
- Insights into cerebral haemodynamics and oxygenation utilising in vivo mural cell imaging and mathematical modelling.Scientific reports · 2018Article
- Deep tissue analysis of distal aqueous drainage structures and contractile features.Scientific reports · 2017Article
- Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta.Journal of visualized experiments : JoVE · 2017Article
- A fluoro-Nissl dye identifies pericytes as distinct vascular mural cells during in vivo brain imaging.Nature neuroscience · 2017Article
- Segregation of Central Ventricular Conduction System Lineages in Early SMA+ Cardiomyocytes Occurs Prior to Heart Tube Formation.Journal of cardiovascular development and disease · 2016Article
- Article
- An α-smooth muscle actin (acta2/αsma) zebrafish transgenic line marking vascular mural cells and visceral smooth muscle cells.PloS one · 2014Article
- Dynamic responses of endothelial cells to changes in blood flow during vascular remodeling of the mouse yolk sac.Development (Cambridge, England) · 2013Article
- Transgenic mice: beyond the knockout.American journal of physiology. Renal physiology · 2011Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Smooth muscle alpha actin (SMA) is a cytoskeletal protein expressed by mesenchymal and smooth muscle cell types, including mural cells (vascular smooth muscle cells and pericytes). Using Bacterial Artificial Chromosome (BAC) recombineering technology, we generated transgenic reporter mice that express a membrane localized cherry red fluorescent protein (mCherry), driven by the full-length SMA promoter and intronic sequences. We determined that the founders and F1 progeny of five independent lines contain 1-3 copies of the mCherry-substituted BAC vector. Furthermore, we characterized the expression of SMA-mCherry in relation to endogenous SMA in the embryo and in adult tissues, and found that the transgenic reporter in each line recapitulated endogenous SMA expression at all time points. We were also able to isolate SMA expressing cells from embryonic tissues using fluorescence-activated cell sorting (FACS). We demonstrated that this marker can be combined with other vital fluorescent reporters and it can be used for live imaging of embryonic cardiodynamics. Therefore, these transgenic mice will be useful for isolating live SMA-expressing cells via FACS and for studying the emergence, behavior, and regulation of SMA-expressing cells, including vascular smooth muscle cells and pericytes throughout embryonic and postnatal development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.