ArticlePloS one2010
A new and fast technique to generate offspring after germ cells transplantation in adult fish: the Nile tilapia (Oreochromis niloticus) model.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 233 citations in OpenAlex.
- Current Advances in Cetacean Semen Cryopreservation and Their Application to Yangtze Finless Porpoise Conservation.Animals : an open access journal from MDPI · 2026Review
- Optimization of Conditions for Cryopreservation of Enriched Spermatogonial Stem Cells in Olive Flounder (Cells · 2026Article
- Intra-ovarian busulfan administration: an effective and relatively safe method for depleting endogenous germ cells in the female marine teleost yellow drum (Nibea albiflora).Fish physiology and biochemistry · 2026Article
- Conservation of Teleost Fishes: Current Status and the Role of Reproductive Technologies.Advances in experimental medicine and biology · 2026Review
- Establishment of Nile Tilapia Primary Cell Culture Methods and In Vitro Cell Knockdown Techniques.Marine biotechnology (New York, N.Y.) · 2024Article
- Evaluation of Female Recipient Infertility and Donor Spermatogonial Purification for Germ Cell Transplantation inAnimals : an open access journal from MDPI · 2024Article
- Post-thaw quality assessment of testicular fragments as a source of spermatogonial cells for surrogate production in the flatfish Solea senegalensis.Fish physiology and biochemistry · 2024Article
- Immune Rejection Mediated byInternational journal of molecular sciences · 2024Article
- Time-efficient germ cell transplantation from goldfish (Animal reproduction · 2024Article
- Generation of surrogate goldfish3 Biotech · 2023Article
- Allogeneic testes transplanted into partially castrated adult medaka (Oryzias latipes) can produce donor-derived offspring by natural mating over a prolonged period.Zoological letters · 2022Article
- Production of functional sperm fromZoological research · 2022Article
- Advantages, Factors, Obstacles, Potential Solutions, and Recent Advances of Fish Germ Cell Transplantation for Aquaculture-A Practical Review.Animals : an open access journal from MDPI · 2022Review
- Preliminary study on testicular germ cell isolation and transplantation in an endangered endemic species Brycon orbignyanus (Characiformes: Characidae).Fish physiology and biochemistry · 2021Article
- Isolation and Characterization of Highly Pure Type A Spermatogonia From Sterlet (Frontiers in cell and developmental biology · 2021Article
- Successful Spermatogonial Stem Cells Transplantation within Pleuronectiformes: First Breakthrough at inter-family Level in Marine Fish.International journal of biological sciences · 2021Article
- Hypoxia-mediated inhibition of cholesterol synthesis leads to disruption of nocturnal sex steroidogenesis in the gonad of koi carp, Cyprinus carpio.Fish physiology and biochemistry · 2020Article
- Cryopreservation of testicular tissue from Murray River Rainbowfish, Melanotaenia fluviatilis.Scientific reports · 2020Article
- Enhanced Enrichment of Medaka Ovarian Germline Stem Cells by a Combination of Density Gradient Centrifugation and Differential Plating.Biomolecules · 2020Article
- MeagreAnimals : an open access journal from MDPI · 2020Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGerm cell transplantation results in fertile recipients and is the only available approach to functionally investigate the spermatogonial stem cell biology in mammals and probably in other vertebrates. In the current study, we describe a novel non-surgical methodology for efficient spermatogonial transplantation into the testes of adult tilapia (O. niloticus), in which endogenous spermatogenesis had been depleted with the cytostatic drug busulfan. METHODOLOGY/PRINCIPAL
findingsUsing two different tilapia strains, the production of fertile spermatozoa with donor characteristics was demonstrated in adult recipient, which also sired progeny with the donor genotype. Also, after cryopreservation tilapia spermatogonial cells were able to differentiate to spermatozoa in the testes of recipient fishes. These findings indicate that injecting germ cells directly into adult testis facilitates and enable fast generation of donor spermatogenesis and offspring compared to previously described methods.
conclusionTherefore, a new suitable methodology for biotechnological investigations in aquaculture was established, with a high potential to improve the production of commercially valuable fish, generate transgenic animals and preserve endangered fish species.
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