ArticleInvestigative ophthalmology & visual science2010
A conditional immortalized mouse muller glial cell line expressing glial and retinal stem cell genes.
Article in Investigative ophthalmology & visual science, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Characterization and neurogenic responses of primary and immortalized Müller glia.Frontiers in cell and developmental biology · 2025Article
- Retinal Inflammation and Reactive Müller Cells: Neurotrophins' Release and Neuroprotective Strategies.Biology · 2024Review
- HIF-1α accumulation in response to transient hypoglycemia may worsen diabetic eye disease.Cell reports · 2023Article
- Human photoreceptors switch from autonomous axon extension to cell-mediated process pulling during synaptic marker redistribution.Cell reports · 2022Article
- Derivation and Characterization of Murine and Amphibian Müller Glia Cell Lines.Translational vision science & technology · 2022Article
- Characteristics of Whale Müller Glia in Primary and Immortalized Cultures.Frontiers in neuroscience · 2022Article
- The "Neuro-Glial-Vascular" Unit: The Role of Glia in Neurovascular Unit Formation and Dysfunction.Frontiers in cell and developmental biology · 2021Review
- VEGF Upregulates EGFR Expression to Stimulate Chemotactic Behaviors in the rMC-1 Model of Müller Glia.Brain sciences · 2020Article
- The Hippo Pathway Blocks Mammalian Retinal Müller Glial Cell Reprogramming.Cell reports · 2019Article
- Substrate Stiffness Influences the Time Dependence of CTGF Protein Expression in Müller Cells.International physiology journal · 2018Article
- Regulation of Stem Cell Properties of Müller Glia by JAK/STAT and MAPK Signaling in the Mammalian Retina.Stem cells international · 2017Review
- TNFa knockdown in the retina promotes cone survival in a mouse model of autosomal dominant retinitis pigmentosa.Biochimica et biophysica acta. Molecular basis of disease · 2017Article
- Effects of 530 nm monochromatic light on basic fibroblast growth factor and transforming growth factor-β1 expression in Müller cells.International journal of ophthalmology · 2015Article
- Low-oxygen culture conditions extend the multipotent properties of human retinal progenitor cells.Tissue engineering. Part A · 2014Article
- AAV-mediated, optogenetic ablation of Müller Glia leads to structural and functional changes in the mouse retina.PloS one · 2013Article
- P53 is required for the developmental restriction in Müller glial proliferation in mouse retina.Glia · 2012Article
- Muller cell expression of genes implicated in proliferative vitreoretinopathy is influenced by substrate elastic modulus.Investigative ophthalmology & visual science · 2012Article
- Eyes on DNA methylation: current evidence for DNA methylation in ocular development and disease.Journal of ocular biology, diseases, and informatics · 2011Article
- Differential expression of neuronal genes in Müller glia in two- and three-dimensional cultures.Investigative ophthalmology & visual science · 2011Article
- A role for DNA methylation in regulation of EphA5 receptor expression in the mouse retina.Vision research · 2011Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
purposeMüller glia have multiple functions in the retina, including synthesis of neurotrophic factors, uptake and metabolism of neurotransmitters, spatial buffering of ions, maintenance of the blood-retinal barrier, and response to injury. A population of Müller glia has some stem cell-like characteristics both in vivo and in vitro. The purpose of this study was to generate and characterize novel Müller glial cell lines from the postnatal mouse retina.
methodsCells were cultured from postnatal day (P) 10 double heterozygous transgenic (H-2K(b)-tsA58/+; HRhoGFP/+) or C57BL/6 mice after papain dissociation. Interferon gamma (IFNγ) induction of the SV40 T-antigen (TAg) was assayed by immunohistochemistry and Western blot analysis. Proliferation was assayed by BrdU uptake and cell counts of calcein AM/ethidium bromide-stained cells. Gene expression was analyzed by RT-PCR and immunohistochemistry.
resultsConditionally immortalized (ImM10 [Immortmouse Müller P10]) and spontaneously immortalized (C57M10 [C57BL/6 Müller P10]) Müller glial cell lines were selected by differential adherence to laminin; both consisted of adherent flat cells with large, diffusely staining nuclei and an epithelial morphology. TAg induction stimulated BrdU uptake by Müller glia in mixed retinal cultures from H-2K(b)-tsA58/+; HRhoGFP/+ mice and increased the proliferation of ImM10 cells. ImM10 and C57M10 cells expressed genes characteristic of Müller glia but not genes characteristic of differentiated retinal neurons. ImM10 cells also expressed retinal stem cell genes.
conclusionsThe ImM10 cell line is a novel, conditionally immortalized Müller glial cell line isolated from the P10 mouse retina that expresses genes characteristic of Müller glial and retinal stem cells.
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