ArticleMolecular biology of the cell2010
Differential roles of transcriptional mediator subunits in regulation of multidrug resistance gene expression in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 49 citations in OpenAlex.
- Candida glabrata YPK2 is a multidrug susceptibility locus.Genetics · 2026Article
- Article
- Loss of PDR3 alters metabolome in response to MCHM, a synthetic hydrotrope.Current genetics · 2025Article
- The role of the Mediator complex in fungal pathogenesis and response to antifungal agents.Essays in biochemistry · 2023Review
- Article
- yEvo: experimental evolution in high school classrooms selects for novel mutations that impact clotrimazole resistance in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2022Article
- SWI/SNF and the histone chaperone Rtt106 drive expression of the Pleiotropic Drug Resistance network genes.Nature communications · 2022Article
- Article
- RPD3 and UME6 are involved in the activation of PDR5 transcription and pleiotropic drug resistance in ρBMC microbiology · 2021Article
- Functional information from clinically-derived drug resistant forms of the Candida glabrata Pdr1 transcription factor.PLoS genetics · 2020Article
- Live-cell assays reveal selectivity and sensitivity of the multidrug response in budding yeast.The Journal of biological chemistry · 2019Article
- Role of Mediator in virulence and antifungal drug resistance in pathogenic fungi.Current genetics · 2019Review
- Yeast AP-1 like transcription factors (Yap) and stress response: a current overview.Microbial cell (Graz, Austria) · 2019Review
- Negative regulation of Candida glabrata Pdr1 by the deubiquitinase subunit Bre5 occurs in a ubiquitin independent manner.Molecular microbiology · 2018Article
- Positive autoregulation and repression of transactivation are key regulatory features of the Candida glabrata Pdr1 transcription factor.Molecular microbiology · 2018Article
- Heterologous Biosynthesis of the Fungal Sesquiterpene Trichodermol inFrontiers in microbiology · 2018Article
- Candida albicans Swi/Snf and Mediator Complexes Differentially Regulate Mrr1-InducedAntimicrobial agents and chemotherapy · 2017Article
- Mediator Tail Module Is Required for Tac1-ActivatedAntimicrobial agents and chemotherapy · 2017Article
- Article
- Evolution of disorder in Mediator complex and its functional relevance.Nucleic acids research · 2016Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The multiprotein transcriptional Mediator complex provides a key link between RNA polymerase II and upstream transcriptional activator proteins. Previous work has established that the multidrug resistance transcription factors Pdr1 and Pdr3 interact with the Mediator component Med15/Gal11 to drive normal levels of expression of the ATP-binding cassette transporter-encoding gene PDR5 in Saccharomyces cerevisiae. PDR5 transcription is induced upon loss of the mitochondrial genome (rho(0) cells) and here we provide evidence that this rho(0) induction is Med15 independent. A search through other known Mediator components determined that Med12/Srb8, a member of the CDK8 Mediator submodule, is required for rho(0) activation of PDR5 transcription. The CDK8 submodule contains the cyclin C homologue (CycC/Srb11), cyclin-dependent kinase Cdk8/Srb10, and the large Med13/Srb9 protein. Loss of these other proteins did not lead to the same block in PDR5 induction. Chromatin immunoprecipitation analyses demonstrated that Med15 is associated with the PDR5 promoter in both rho(+) and rho(0), whereas Med12 recruitment to this target promoter is highly responsive to loss of the mitochondrial genome. Coimmunoprecipitation experiments revealed that association of Pdr3 with Med12 can only be detected in rho(0) cells. These experiments uncover the unique importance of Med12 in activated transcription of PDR5 seen in rho(0) cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.