ArticleNucleic acids research2010
Northern blot detection of endogenous small RNAs (approximately14 nt) in bacterial total RNA extracts.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- 6S-1 pRNA 9-mers are a prominent length species during outgrowth ofRNA biology · 2025Article
- Processing and decay of 6S-1 and 6S-2 RNAs inRNA (New York, N.Y.) · 2023Article
- Involvement ofInternational journal of molecular sciences · 2022Article
- A novel mechanism of ribonuclease regulation: GcvB and Hfq stabilize the mRNA that encodes RNase BN/Z during exponential phase.The Journal of biological chemistry · 2019Article
- Article
- Purification of cross-linked RNA-protein complexes by phenol-toluol extraction.Nature communications · 2019Article
- Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins Using SYBR Green-Based qRT-PCR.Methods in molecular biology (Clifton, N.J.) · 2018Article
- 6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype.RNA biology · 2017Article
- Removing bias against short sequences enables northern blotting to better complement RNA-seq for the study of small RNAs.Nucleic acids research · 2017Article
- Growth Phase-dependent Variation of RNase BN/Z Affects Small RNAs: REGULATION OF 6S RNA.The Journal of biological chemistry · 2016Article
- Plasmid pPCP1-derived sRNA HmsA promotes biofilm formation of Yersinia pestis.BMC microbiology · 2016Article
- Large-scale identification of small noncoding RNA with strand-specific deep sequencing and characterization of a novel virulence-related sRNA in Brucella melitensis.Scientific reports · 2016Article
- Bacillus subtilis 6S-2 RNA serves as a template for short transcripts in vivo.RNA (New York, N.Y.) · 2016Article
- Studies on Transcriptional Incorporation of 5'-N-Triphosphates of 5'-Amino-5'-Deoxyribonucleosides.PloS one · 2016Article
- Identification of a Novel Small Non-Coding RNA Modulating the Intracellular Survival of Brucella melitensis.Frontiers in microbiology · 2015Article
- Genomewide comparison and novel ncRNAs of Aquificales.BMC genomics · 2014Article
- Rapid degradation of Hfq-free RyhB in Yersinia pestis by PNPase independent of putative ribonucleolytic complexes.BioMed research international · 2014Article
- 6S-1 RNA function leads to a delay in sporulation in Bacillus subtilis.Journal of bacteriology · 2013Article
- Determination of sRNA expressions by RNA-seq in Yersinia pestis grown in vitro and during infection.PloS one · 2013Article
- A pRNA-induced structural rearrangement triggers 6S-1 RNA release from RNA polymerase in Bacillus subtilis.The EMBO journal · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we describe a northern blot procedure that allows the detection of endogenous RNAs as small as approximately 14 nt in total RNA extracts from bacteria. RNAs that small and as part of total bacterial RNA extracts usually escape detection by northern blotting. The approach combines LNA probes 5'-digoxigenin-endlabeled for non-radioactive probe detection with 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide-mediated chemical crosslinking of RNAs to nylon membranes, and necessitates the use of native PAGE either with the TBE or MOPS buffer system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.