ArticleThe Journal of biological chemistry2010
Interaction of cytosolic glutamine synthetase of soybean root nodules with the C-terminal domain of the symbiosome membrane nodulin 26 aquaglyceroporin.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 71 citations in OpenAlex.
- Uclacyanin MtUC1 Is Involved in the Regulation of Nodule Senescence in Medicago truncatula.Molecular plant pathology · 2025Article
- Characterization of the Glutamine Synthetase Gene Family in Wheat (International journal of molecular sciences · 2025Article
- Nutrient-dependent regulation of symbiotic nitrogen fixation in legumes.Horticulture research · 2025Article
- M. mazei glutamine synthetase and glutamine synthetase-GlnK1 structures reveal enzyme regulation by oligomer modulation.Nature communications · 2023Article
- The Importance of Microorganisms for Sustainable Agriculture-A Review.Metabolites · 2022Review
- Molecular dissection of the glutamine synthetase-GlnR nitrogen regulatory circuitry in Gram-positive bacteria.Nature communications · 2022Article
- Aquaporin ion conductance properties defined by membrane environment, protein structure, and cell physiology.Biophysical reviews · 2022Review
- A roadmap of plant membrane transporters in arbuscular mycorrhizal and legume-rhizobium symbioses.Plant physiology · 2021Review
- Diazotrophs for Lowering Nitrogen Pollution Crises: Looking Deep Into the Roots.Frontiers in microbiology · 2021Review
- Dynamic Control of the High-Affinity Iron Uptake Complex in Root Epidermal Cells.Plant physiology · 2020Article
- Review
- Is Nitrogen a Key Determinant of Water Transport and Photosynthesis in Higher Plants Upon Drought Stress?Frontiers in plant science · 2018Review
- Symbiotic Nitrogen Fixation and the Challenges to Its Extension to Nonlegumes.Applied and environmental microbiology · 2016Review
- Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana.The Plant cell · 2016Article
- The Roles of Aquaporins in Plant Stress Responses.Journal of developmental biology · 2016Review
- Proteomic analysis of the soybean symbiosome identifies new symbiotic proteins.Molecular & cellular proteomics : MCP · 2015Article
- Glutamine synthetase in Medicago truncatula, unveiling new secrets of a very old enzyme.Frontiers in plant science · 2015Review
- A nitrogen-dependent switch in the high affinity ammonium transport in Medicago truncatula.Plant molecular biology · 2014Article
- Matching the supply of bacterial nutrients to the nutritional demand of the animal host.Proceedings. Biological sciences · 2014Article
- An RNA sequencing transcriptome analysis reveals novel insights into molecular aspects of the nitrate impact on the nodule activity of Medicago truncatula.Plant physiology · 2014Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nodulin 26 (nod26) is a major intrinsic protein that constitutes the major protein component on the symbiosome membrane (SM) of N(2)-fixing soybean nodules. Functionally, nod26 forms a low energy transport pathway for water, osmolytes, and NH(3) across the SM. Besides their transport functions, emerging evidence suggests that high concentrations of major intrinsic proteins on membranes provide interaction and docking targets for various cytosolic proteins. Here it is shown that the C-terminal domain peptide of nod26 interacts with a 40-kDa protein from soybean nodule extracts, which was identified as soybean cytosolic glutamine synthetase GS(1)beta1 by mass spectrometry. Fluorescence spectroscopy assays show that recombinant soybean GS(1)beta1 binds the nod26 C-terminal domain with a 1:1 stoichiometry (K(d) = 266 nm). GS(1)beta1 also binds to isolated SMs, and this binding can be blocked by preincubation with the C-terminal peptide of nod26. In vivo experiments using either a split ubiquitin yeast two-hybrid system or bimolecular fluorescence complementation show that the four cytosolic GS isoforms expressed in soybean nodules interact with full-length nod26. The binding of GS, the principal ammonia assimilatory enzyme, to the conserved C-terminal domain of nod26, a transporter of NH(3), is proposed to promote efficient assimilation of fixed nitrogen, as well as prevent potential ammonia toxicity, by localizing the enzyme to the cytosolic side of the symbiosome membrane.
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