ArticleBMC plant biology2010
Regulation of Arabidopsis defense responses against Spodoptera littoralis by CPK-mediated calcium signaling.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed.
- The zig-zag model of molecular defense and counterdefense in plant-herbivore interactions.Planta · 2026Review
- The CPK3-mediated cytosolic-nuclear translocation of bHLH107 recruits HY5 to regulate the CuJournal of advanced research · 2026Article
- Transcriptome and small RNA sequencings reveal the response of tobacco to aphid infestation.BMC genomics · 2025Article
- GmIQD63 functions as a novel GmCDPK38-interacting protein in soybean defense against the common cutworm.Planta · 2025Article
- Identification, characterization and expression analysis of the CDPK family in kelp Saccharina japonica.BMC genomics · 2025Article
- Interdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underlieseLife · 2025Article
- Comparative translational reprogramming ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Arabidopsis Calcium Dependent Protein Kinase 3, and Its Orthologues OsCPK1, OsCPK15, and AcCPK16, Are Involved in Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2025Article
- Calcium-dependent protein kinases in plant immunity: from calcium signaling to network integration.Frontiers in plant science · 2025Review
- Intricate intracellular kinase network regulates the Spodoptera lituta-derived elicitor response signaling in Arabidopsis.Journal of plant research · 2025Article
- Applications of the wheat germ cell-free protein synthesis system in plant biochemical studies.Plant biotechnology (Tokyo, Japan) · 2024Article
- CRISPR-Cas9-mediated construction of a cotton CDPK mutant library for identification of insect-resistance genes.Plant communications · 2024Article
- OsCPK12 phosphorylates OsCATA and OsCATC to regulate HPlant communications · 2024Article
- Calcium imaging: a technique to monitor calcium dynamics in biological systems.Physiology and molecular biology of plants : an international journal of functional plant biology · 2023Review
- Cis-regulatory elements and transcription factors related to auxin signaling in the streptophyte algae Klebsormidium nitens.Scientific reports · 2023Article
- Cytoplasmic Kinase Network Mediates Defense Response toPlants (Basel, Switzerland) · 2023Article
- RNA-Seq Dissects Incomplete Activation of Phytoalexin Biosynthesis by the Soybean Transcription Factors GmMYB29A2 and GmNAC42-1.Plants (Basel, Switzerland) · 2023Article
- The Roles of CDPKs as a Convergence Point of Different Signaling Pathways in Maize Adaptation to Abiotic Stress.International journal of molecular sciences · 2023Review
- The calcium connection: exploring the intricacies of calcium signaling in plant-microbe interactions.Frontiers in plant science · 2023Review
- The Re-Localization of Proteins to or Away from Membranes as an Effective Strategy for Regulating Stress Tolerance in Plants.Membranes · 2022Review
9 more citing papers are in PubMed but not listed here.
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15 authors.
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Abstract
backgroundPlant Ca2+ signals are involved in a wide array of intracellular signaling pathways after pest invasion. Ca2+-binding sensory proteins such as Ca2+-dependent protein kinases (CPKs) have been predicted to mediate the signaling following Ca2+ influx after insect herbivory. However, until now this prediction was not testable.
resultsTo investigate the roles CPKs play in a herbivore response-signaling pathway, we screened the characteristics of Arabidopsis CPK mutants damaged by a feeding generalist herbivore, Spodoptera littoralis. Following insect attack, the cpk3 and cpk13 mutants showed lower transcript levels of plant defensin gene PDF1.2 compared to wild-type plants. The CPK cascade was not directly linked to the herbivory-induced signaling pathways that were mediated by defense-related phytohormones such as jasmonic acid and ethylene. CPK3 was also suggested to be involved in a negative feedback regulation of the cytosolic Ca2+ levels after herbivory and wounding damage. In vitro kinase assays of CPK3 protein with a suite of substrates demonstrated that the protein phosphorylates transcription factors (including ERF1, HsfB2a and CZF1/ZFAR1) in the presence of Ca2+. CPK13 strongly phosphorylated only HsfB2a, irrespective of the presence of Ca2+. Furthermore, in vivo agroinfiltration assays showed that CPK3-or CPK13-derived phosphorylation of a heat shock factor (HsfB2a) promotes PDF1.2 transcriptional activation in the defense response.
conclusionsThese results reveal the involvement of two Arabidopsis CPKs (CPK3 and CPK13) in the herbivory-induced signaling network via HsfB2a-mediated regulation of the defense-related transcriptional machinery. This cascade is not involved in the phytohormone-related signaling pathways, but rather directly impacts transcription factors for defense responses.
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