ArticleCytotechnology2010
Establishment of a turbot fin cell line and its susceptibility to turbot reddish body iridovirus.
Article in Cytotechnology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
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- Megalocytivirus and Other Members of the FamilyViruses · 2023Review
- Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus).In vitro cellular & developmental biology. Animal · 2023Article
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- Development of a gene-deleted live attenuated candidate vaccine against fish virus (ISKNV) with low pathogenicity and high protection.iScience · 2021Article
- Megalocytiviruses in ornamental fish: A review.Veterinary world · 2020Review
- Establishment and characterization of a fibroblast-like cell line from the muscle of turbot (Scophthalmus maximus L.).Fish physiology and biochemistry · 2019Article
- Article
- Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale.Fish physiology and biochemistry · 2015Article
- RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from Dicentrarchus labrax.Cytotechnology · 2014Article
- Cytotoxic effects of betaxolol on healthy corneal endothelial cells both in vitro and in vivo.International journal of ophthalmology · 2014Article
- Establishment and characterization of a fin cell line from blunt snout bream, Megalobrama amblycephala.Fish physiology and biochemistry · 2013Article
- Local anesthetic lidocaine induces apoptosis in human corneal stromal cells in vitro.International journal of ophthalmology · 2013Article
- Establishment, characterization and immortalization of a fibroblast cell line from the Chinese red belly toad Bombina maxima skin.Cytotechnology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A turbot, Scophthalmus maximus, fin (TF) cell line was established and susceptibility to turbot reddish body iridovirus (TRBIV) was determined in this study. Primary culture of TF cells was initiated from fin tissue pieces partially digested with trypsin, collagenase II and hyaluronidase. Digested tissue pieces were cultured at 24 degrees C in Leibovitz-15 medium (pH 7.2), supplemented with 20% fetal bovine serum, carboxymethyl chitosan, N-acetylglucosamine hydrochloride, basic fibroblast growth factor and epidermal growth factor. The cultured TF cells, in fibroblast shape, proliferated to 100% confluency 50 days later. A TF cell line, with a population doubling time of 45.6 h at passage 80, has been established and subcultured to passage 133. Chromosome analyses indicated that the TF cells exhibited chromosomal aneuploidy with a modal chromosome number of 44 which displayed the normal diploid karyotype of S. maximus at least up to passage 80. TRBIV susceptibility testing demonstrated that cytopathic effect and propagated viral particles were observed in TF cells after TRBIV infection. In conclusion, a continuous TRBIV susceptible TF cell line has been established successfully, and the cell line may serve as a valuable tool for studies of cell-virus interactions and has applications for different kinds of cytotechnological studies as well.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.