Evidence map›Paper›PMID 20502665›Full record

ArticlePloS one2010

Sequencing, analysis, and annotation of expressed sequence tags for Camelus dromedarius.

Abdulaziz M Al-Swailem, Maher M Shehata, Faisel M Abu-Duhier, Essam J Al-Yamani, Khalid A Al-Busadah, Mohammed S Al-Arawi, Ali Y Al-Khider, Abdullah N Al-Muhaimeed, Fahad H Al-Qahtani, Manee M Manee and 6 more

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed, 1 pooled it
5.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.

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  18. Evaluating the roles of directed breeding and gene flow in animal domestication.Proceedings of the National Academy of Sciences of the United States of America · 2014
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 3 countries.

Abdulaziz M Al-SwailemBiotechnology Research Center, Natural Resources and Environment Research Institute, King Abdulaziz City for Science and Technology, Riyadh, Saudi Arabia.
Maher M Shehata
Faisel M Abu-Duhier
Essam J Al-Yamani
Khalid A Al-Busadah
Mohammed S Al-Arawi
Ali Y Al-Khider
Abdullah N Al-Muhaimeed
Fahad H Al-Qahtani
Manee M Manee
Badr M Al-Shomrani
Saad M Al-Qhtani
Amer S Al-Harthi
Kadir C Akdemir
Mehmet S Inan
Hasan H Otu
King Abdulaziz City for Science and Technology · SABeth Israel Deaconess Hospital · USKing Faisal University · SA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Despite its economical, cultural, and biological importance, there has not been a large scale sequencing project to date for Camelus dromedarius. With the goal of sequencing complete DNA of the organism, we first established and sequenced camel EST libraries, generating 70,272 reads. Following trimming, chimera check, repeat masking, cluster and assembly, we obtained 23,602 putative gene sequences, out of which over 4,500 potentially novel or fast evolving gene sequences do not carry any homology to other available genomes. Functional annotation of sequences with similarities in nucleotide and protein databases has been obtained using Gene Ontology classification. Comparison to available full length cDNA sequences and Open Reading Frame (ORF) analysis of camel sequences that exhibit homology to known genes show more than 80% of the contigs with an ORF>300 bp and approximately 40% hits extending to the start codons of full length cDNAs suggesting successful characterization of camel genes. Similarity analyses are done separately for different organisms including human, mouse, bovine, and rat. Accompanying web portal, CAGBASE (http://camel.kacst.edu.sa/), hosts a relational database containing annotated EST sequences and analysis tools with possibility to add sequences from public domain. We anticipate our results to provide a home base for genomic studies of camel and other comparative studies enabling a starting point for whole genome sequencing of the organism.

Indexed as

Expressed Sequence TagsAnimalsBase SequenceCattleCluster AnalysisDNA, ComplementaryGene Regulatory NetworksHumansMiceOpen Reading FramesRatsSequence Analysis, DNASequence Homology, Nucleic AcidSignal TransductionDNA, Complementary

Identifiers

PMID20502665
PMCPMC2873428
OpenAlexW2021209351

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.