ArticlePloS one2010
Loss of the heparan sulfate sulfotransferase, Ndst1, in mammary epithelial cells selectively blocks lobuloalveolar development in mice.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Space Environment Impacts Homeostasis: Exposure to Spaceflight Alters Mammary Gland Transportome Genes.Biomolecules · 2023Article
- Physiology and Pathophysiology of Heparan Sulfate in Animal Models: Its Biosynthesis and Degradation.International journal of molecular sciences · 2022Review
- Characterization of Gene Expression Signatures for the Identification of Cellular Heterogeneity in the Developing Mammary Gland.Journal of mammary gland biology and neoplasia · 2021Article
- Three-Dimensional Models as a New Frontier for Studying the Role of Proteoglycans in the Normal and Malignant Breast Microenvironment.Frontiers in cell and developmental biology · 2020Review
- The Roles of Indian Hedgehog Signaling in TMJ Formation.International journal of molecular sciences · 2019Review
- Hepatic heparan sulfate is a master regulator of hepcidin expression and iron homeostasis in human hepatocytes and mice.The Journal of biological chemistry · 2019Article
- Patterned cell and matrix dynamics in branching morphogenesis.The Journal of cell biology · 2017Review
- The function of heparan sulfate during branching morphogenesis.Matrix biology : journal of the International Society for Matrix Biology · 2017Review
- Glycomics: revealing the dynamic ecology and evolution of sugar molecules.Journal of proteomics · 2016Review
- Role of Deacetylase Activity of N-Deacetylase/N-Sulfotransferase 1 in Forming N-Sulfated Domain in Heparan Sulfate.The Journal of biological chemistry · 2015Article
- Growth factor-heparan sulfate "switches" regulating stages of branching morphogenesis.Pediatric nephrology (Berlin, Germany) · 2014Review
- Glycan-based biomarkers for mucopolysaccharidoses.Molecular genetics and metabolism · 2014Review
- Human genetic disorders and knockout mice deficient in glycosaminoglycan.BioMed research international · 2014Review
- miR-191: an emerging player in disease biology.Frontiers in genetics · 2014Review
- Heparan sulfate and heparanase as modulators of breast cancer progression.BioMed research international · 2013Review
- N-sulfation of heparan sulfate regulates early branching events in the developing mammary gland.The Journal of biological chemistry · 2012Article
- Heparan sulfate biosynthesis: regulation and variability.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Review
- The glomerular basement membrane as a model system to study the bioactivity of heparan sulfate glycosaminoglycans.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2012Review
- Stage-dependent regulation of mammary ductal branching by heparan sulfate and HGF-cMet signaling.Developmental biology · 2011Article
- Heparan sulfate proteoglycans.Cold Spring Harbor perspectives in biology · 2011Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
backgroundConsiderable evidence indicates that heparan sulfate is essential for the development of tissues consisting of branching ducts and tubules. However, there are few examples where specific sulfate residues regulate a specific stage in the formation of such tissues. METHODOLOGY/PRINCIPAL
findingsWe examined the role of heparan sulfation in mammary gland branching morphogenesis, lactation and lobuloalveolar development by inactivation of heparan sulfate GlcNAc N-deacetylase/N-sulfotransferase genes (Ndst) in mammary epithelial cells using the Cre-loxP system. Ndst1 deficiency resulted in an overall reduction in glucosamine N-sulfation and decreased binding of FGF to mammary epithelial cells in vitro and in vivo. Mammary epithelia lacking Ndst1 underwent branching morphogenesis, filling the gland with ductal tissue by sexual maturity to the same extent as wildtype epithelia. However, lobuloalveolar expansion did not occur in Ndst1-deficient animals, resulting in insufficient milk production to nurture newly born pups. Lactational differentiation of isolated mammary epithelial cells occurred appropriately via stat5 activation, further supporting the notion that the lack of milk production was due to lack of expansion of the lobuloalveoli. CONCLUSIONS/SIGNIFICANCE: These findings demonstrate a selective, highly penetrant, cell autonomous effect of Ndst1-mediated sulfation on lobuloalveolar development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.