ArticleThe Journal of biological chemistry2010
Protein Radical Formation Resulting from Eosinophil Peroxidase-catalyzed Oxidation of Sulfite.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 56 citations in OpenAlex.
- Simple, Fast, and Ultrasensitive Fluorescent Detection of Peroxymonosulfate in Chemical, Enzymatic, and Cellular Systems.Analytical chemistry · 2026Article
- Sulfur Dioxide in Food: A Review of Its Preservative Mechanisms, Safety Concerns, and the Rise of Smart Detection Systems.Comprehensive reviews in food science and food safety · 2026Review
- Interactions of reactive sulfur species with metalloproteins.Redox biology · 2023Review
- Application of a novel heterogeneous sulfite activation with copper(i) sulfide (CuRSC advances · 2022Article
- Synthesis, Characterization, and Application of a Highly Hydrophilic Triarylmethyl Radical for Biomedical EPR.The Journal of organic chemistry · 2020Article
- Eosinophil peroxidase over-expression predicts the clinical outcome of patients with primary lung adenocarcinoma.Journal of Cancer · 2019Article
- Sulfite-induced protein radical formation in LPS aerosol-challenged mice: Implications for sulfite sensitivity in human lung disease.Redox biology · 2018Article
- Diet Modulates Adipose Tissue Oxidative Stress in a Murine Acute Chagas Model.JSM atherosclerosis · 2017Article
- Imaging free radicals in organelles, cells, tissue, and in vivo with immuno-spin trapping.Redox biology · 2016Review
- Sulfite Oxidase Activity of Cytochrome c: Role of Hydrogen Peroxide.Biochemistry and biophysics reports · 2016Article
- Globins Scavenge Sulfur Trioxide Anion Radical.The Journal of biological chemistry · 2015Article
- Immuno-spin trapping from biochemistry to medicine: advances, challenges, and pitfalls. Focus on protein-centered radicals.Biochimica et biophysica acta · 2014Review
- Thiocyanate potentiates antimicrobial photodynamic therapy: in situ generation of the sulfur trioxide radical anion by singlet oxygen.Free radical biology & medicine · 2013Article
- Investigation of spin-trapping artifacts formed by the Forrester-Hepburn mechanism.Free radical biology & medicine · 2013Article
- Sulfite-mediated oxidation of myeloperoxidase to a free radical: immuno-spin trapping detection in human neutrophils.Free radical biology & medicine · 2013Article
- Development of immunoblotting techniques for DNA radical detection.Free radical biology & medicine · 2013Article
- In vivo imaging of immuno-spin trapped radicals with molecular magnetic resonance imaging in a diabetic mouse model.Diabetes · 2012Article
- Detection of Ras GTPase protein radicals through immuno-spin trapping.Free radical biology & medicine · 2012Article
- Potential implication of the chemical properties and bioactivity of nitrone spin traps for therapeutics.Future medicinal chemistry · 2012Review
- Oxidative balance and colon and rectal cancer: interaction of lifestyle factors and genes.Mutation research · 2012Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Eosinophil peroxidase (EPO) is an abundant heme protein in eosinophils that catalyzes the formation of cytotoxic oxidants implicated in asthma, allergic inflammatory disorders, and cancer. It is known that some proteins with peroxidase activity (horseradish peroxidase and prostaglandin hydroperoxidase) can catalyze oxidation of bisulfite (hydrated sulfur dioxide), leading to the formation of sulfur trioxide anion radical ((.)SO(3)(-)). This free radical further reacts with oxygen to form peroxymonosulfate anion radical ((-)O(3)SOO(.)) and the very reactive sulfate anion radical (SO(4)()), which is nearly as strong an oxidant as the hydroxyl radical. However, the ability of EPO to generate reactive sulfur radicals has not yet been reported. Here we demonstrate that eosinophil peroxidase/H(2)O(2) is able to oxidize bisulfite, ultimately forming the sulfate anion radical (SO(4)()), and that these reactive intermediates can oxidize target proteins to protein radicals, thereby initiating protein oxidation. We used immuno-spin trapping and confocal microscopy to study protein oxidation by EPO/H(2)O(2) in the presence of bisulfite in a pure enzymatic system and in human promyelocytic leukemia HL-60 clone 15 cells, maturated to eosinophils. Polyclonal antiserum raised against the spin trap 5,5-dimethyl-1-pyrroline N-oxide (DMPO) detected the presence of DMPO covalently attached to the proteins resulting from the DMPO trapping of protein free radicals. We found that sulfite oxidation mediated by EPO/H(2)O(2) induced the formation of radical-derived DMPO spin-trapped human serum albumin and, to a lesser extent, of DMPO-EPO. These studies suggest that EPO-dependent oxidative damage may play a role in tissue injury in bisulfite-exacerbated eosinophilic inflammatory disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.