ArticleAmerican journal of physiology. Gastrointestinal and liver physiology2009
Role of thin-filament regulatory proteins in relaxation of colonic smooth muscle contraction.
Article in American journal of physiology. Gastrointestinal and liver physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 14 citations in OpenAlex.
- Pleiotropic microRNA-21 in pulmonary remodeling: novel insights for molecular mechanism and present advancements.Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology · 2019Review
- Bioengineering functional human sphincteric and non-sphincteric gastrointestinal smooth muscle constructs.Methods (San Diego, Calif.) · 2016Article
- Amino acid mutations in the caldesmon COOH-terminal functional domain increase force generation in bladder smooth muscle.American journal of physiology. Renal physiology · 2013Article
- Uterine smooth muscle S-nitrosylproteome in pregnancy.Molecular pharmacology · 2012Article
- Role of myosin light chain kinase in regulation of basal blood pressure and maintenance of salt-induced hypertension.American journal of physiology. Heart and circulatory physiology · 2011Article
- Phosphorylated HSP20 modulates the association of thin-filament binding proteins: caldesmon with tropomyosin in colonic smooth muscle.American journal of physiology. Gastrointestinal and liver physiology · 2010Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Coordinated regulation of smooth muscle contraction and relaxation is required for colonic motility. Contraction is associated with phosphorylation of myosin light chain (MLC(20)) and interaction of actin with myosin. Thin-filament regulation of actomyosin interaction is modulated by two actin-binding regulatory proteins: tropomyosin (TM) and caldesmon (CaD). TM and CaD are known to play crucial role in actomyosin interaction promoting contraction. Contraction is associated with phosphorylation of the small heat shock protein HSP27, concomitant with the phosphorylation of TM and CaD. Phosphorylation of HSP27 is attributed as being the prime modulator of thin-filament regulation of contraction. Preincubation of colonic smooth muscle cells (CSMC) with the relaxant neurotransmitter vasoactive intestinal peptide (VIP) showed inhibition in phosphorylation of HSP27 (ser78). Attenuation of HSP27 phosphorylation can result in modulation of thin-filament-mediated regulation of contraction leading to relaxation; thus the role of thin-filament regulatory proteins in a relaxation milieu was investigated. Preincubation of CSMC with VIP exhibited a decrease in phosphorylation of TM and CaD. Furthermore, CSMC preincubated with VIP showed a reduced association of TM with HSP27 and with phospho-HSP27 (ser78) whereas there was reduced dissociation of TM from CaD and from phospho-CaD. We thus propose that, in addition to alteration in phosphorylation of MLC(20), relaxation is associated with alterations in thin-filament-mediated regulation that results in termination of contraction.
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