ArticleThe EMBO journal1991
vac2: a yeast mutant which distinguishes vacuole segregation from Golgi-to-vacuole protein targeting.
Article in The EMBO journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- CRISPR knockout screen implicates three genes in lysosome function.Scientific reports · 2019Article
- Target of rapamycin signaling mediates vacuolar fragmentation.Current genetics · 2017Review
- The yeast lysosome-like vacuole: endpoint and crossroads.Biochimica et biophysica acta · 2009Review
- Candida albicans VAC8 is required for vacuolar inheritance and normal hyphal branching.Eukaryotic cell · 2006Article
- Vac8p, a vacuolar protein with armadillo repeats, functions in both vacuole inheritance and protein targeting from the cytoplasm to vacuole.The Journal of cell biology · 1998Article
- Vacuole biogenesis in Saccharomyces cerevisiae: protein transport pathways to the yeast vacuole.Microbiology and molecular biology reviews : MMBR · 1998Review
- I2B is a small cytosolic protein that participates in vacuole fusion.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- The yeast gene, MDM20, is necessary for mitochondrial inheritance and organization of the actin cytoskeleton.The Journal of cell biology · 1997Article
- A heterodimer of thioredoxin and I(B)2 cooperates with Sec18p (NSF) to promote yeast vacuole inheritance.The Journal of cell biology · 1997Article
- Actin and myosin function in directed vacuole movement during cell division in Saccharomyces cerevisiae.The Journal of cell biology · 1996Article
- Vacuole partitioning during meiotic division in yeast.Genetics · 1996Article
- Multiple classes of yeast mutants are defective in vacuole partitioning yet target vacuole proteins correctly.Molecular biology of the cell · 1996Article
- Homotypic vacuole fusion requires Sec17p (yeast alpha-SNAP) and Sec18p (yeast NSF).The EMBO journal · 1996Article
- Thioredoxin is required for vacuole inheritance in Saccharomyces cerevisiae.The Journal of cell biology · 1996Article
- The GTPase Ypt7p of Saccharomyces cerevisiae is required on both partner vacuoles for the homotypic fusion step of vacuole inheritance.The EMBO journal · 1995Article
- A truncated form of the Pho80 cyclin redirects the Pho85 kinase to disrupt vacuole inheritance in S. cerevisiae.The Journal of cell biology · 1995Article
- Determination of four biochemically distinct, sequential stages during vacuole inheritance in vitro.The Journal of cell biology · 1994Article
- G-protein ligands inhibit in vitro reactions of vacuole inheritance.The Journal of cell biology · 1994Article
- Morphological classification of the yeast vacuolar protein sorting mutants: evidence for a prevacuolar compartment in class E vps mutants.Molecular biology of the cell · 1992Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have isolated four yeast mutants that are unable to partition maternal vacuoles into growing buds. Three of these vacuole segregation (vac) mutants also mislocalize the vacuolar protease carboxypeptidase Y (CPY) to the cell surface, a phenotype previously reported for vac strains. A fourth mutant, vac2-1, exhibits a temperature-sensitive defect in vacuole segregation but does not show a defect in protein targeting from the Golgi apparatus to the vacuole. Haploid vac2-1 cells grown at the non-permissive temperature do not secrete CPY or a second vacuolar protease, proteinase A (PrA). Furthermore, newly synthesized precursors of CPY are converted to mature forms with similar kinetics in both vac2-1 and wild-type cells. In addition, invertase is secreted normally from vac2-1 cells, indicating that post-Golgi steps in the secretory pathway are not blocked in this mutant. These results suggest that VAC2 function is necessary for vacuole division and segregation in yeast but is not involved in vacuole protein sorting events at the Golgi apparatus.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.