ArticleBMC genomics2010
Genomic profiling of tumor initiating prostatospheres.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 71 citations in OpenAlex.
- Review
- ELF4 contributes to esophageal squamous cell carcinoma growth and metastasis by augmenting cancer stemness via FUT9.Acta biochimica et biophysica Sinica · 2024Article
- Evidence that cervical cancer cells cultured as tumorspheres maintain high CD73 expression and increase their protumor characteristics through TGF-β production.Cell biochemistry and function · 2022Article
- Suppression of tumor metastasis by a RECK-activating small molecule.Scientific reports · 2022Article
- CXCL12γ induces human prostate and mammary gland development.The Prostate · 2020Article
- STAT3 inhibition with galiellalactone effectively targets the prostate cancer stem-like cell population.Scientific reports · 2020Article
- Epithelial tumor suppressor ELF3 is a lineage-specific amplified oncogene in lung adenocarcinoma.Nature communications · 2019Article
- microRNA-32-5p targets KLF2 to promote gastric cancer by activating PI3K/AKT signaling pathway.American journal of translational research · 2019Article
- In-Silico Integration Approach to Identify a Key miRNA Regulating a Gene Network in Aggressive Prostate Cancer.International journal of molecular sciences · 2018Article
- Could ALDH2Oncotarget · 2018Review
- Krüppel-like factor 2 suppresses human gastric tumorigenesis through inhibiting PTEN/AKT signaling.Oncotarget · 2017Article
- Cancer cell-soluble factors reprogram mesenchymal stromal cells to slow cycling, chemoresistant cells with a more stem-like state.Stem cell research & therapy · 2017Article
- Roles and regulations of the ETS transcription factor ELF4/MEF.Journal of molecular cell biology · 2017Review
- The role of prostate tumor overexpressed 1 in cancer progression.Oncotarget · 2017Review
- Genotypes of cancer stem cells characterized by epithelial-to-mesenchymal transition and proliferation related functions.Scientific reports · 2016Article
- DNMT1 Regulates Epithelial-Mesenchymal Transition and Cancer Stem Cells, Which Promotes Prostate Cancer Metastasis.Neoplasia (New York, N.Y.) · 2016Article
- The marrow niche controls the cancer stem cell phenotype of disseminated prostate cancer.Oncotarget · 2016Article
- Cryptotanshinone targets tumor-initiating cells through down-regulation of stemness genes expression.Oncology letters · 2016Article
- Endogenous GAS6 and Mer receptor signaling regulate prostate cancer stem cells in bone marrow.Oncotarget · 2016Article
- KLF2 is downregulated in pancreatic ductal adenocarcinoma and inhibits the growth and migration of cancer cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundThe cancer stem cell (CSC) hypothesis proposes that a population of tumor cells bearing stem cell properties is responsible for the origin and maintenance of tumors. Normal and cancer stem cells possess the ability to grow in vitro as self-renewing spheres, but the molecular basis of this phenotype remains largely unknown. We intended to establish a comprehensive culture system to grow prostatospheres (PSs) from both cancer cell lines and patient tumors. We then used gene expression microarrays to gain insight on the molecular pathways that sustain the PS tumor initiating cell (TIC) phenotype.
resultsTraditional stem cell medium (SCM) supplemented with KnockoutSR (KO) allows the propagation of monoclonal PSs from cell lines and primary cells. PSs display gene expression and tumorigenicity hallmarks of TICs. Gene expression analysis defined a gene signature composed of 66 genes that characterize LNCaP and patient PSs. This set includes novel prostate TIC growth factors (NRP1, GDF1, JAG1), proteins implicated in cell adhesion and cytoskeletal maintenance, transcriptional regulators (MYCBP, MYBL1, ID1, ID3, FOS, ELF3, ELF4, KLF2, KLF5) and factors involved in protein biosynthesis and metabolism. Meta-analysis in Oncomine reveals that some of these genes correlate with prostate cancer status and/or progression. Reporter genes and inhibitors indicate that the Notch pathway contributes to prostatosphere growth.
conclusionsWe have developed a model for the culture of PSs, and provide a genomic profile that support CSCs identity. This signature identifies novel markers and pathways that are predicted to correlate with prostate cancer evolution.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.