ArticleCytopathology : official journal of the British Society for Clinical Cytology2011
PINCH-2 expression in cancers involving serosal effusions using quantitative PCR.
Article in Cytopathology : official journal of the British Society for Clinical Cytology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 13 citations in OpenAlex.
- Article
- Migfilin, α-parvin and β-parvin are differentially expressed in ovarian serous carcinoma effusions, primary tumors and solid metastases.Gynecologic oncology · 2013Article
- AZGP1 and SPDEF mRNA expression differentiates breast carcinoma from ovarian serous carcinoma.Virchows Archiv : an international journal of pathology · 2013Article
- Rab25 is overexpressed in Müllerian serous carcinoma compared to malignant mesothelioma.Virchows Archiv : an international journal of pathology · 2012Article
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6 authors at 3 institutions in 2 countries.
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Abstract
objectiveThe PINCH-2 gene was previously shown to be overexpressed in malignant mesothelioma compared with ovarian/peritoneal serous carcinoma in Affymetrix array analysis. The objective of the present study was to validate this finding at the mRNA and protein level.
methodsEffusions (n = 91; 71 ovarian and 10 breast carcinomas, 10 malignant mesotheliomas) were assayed for PINCH-2 mRNA expression using quantitative PCR. PINCH-2 protein expression was analysed in 37 effusions using flow cytometry.
resultsQuantitative PCR analysis showed significantly higher PINCH-2 mRNA levels in mesotheliomas compared with carcinomas (P = 0.004). Values of <10 copies were found exclusively in carcinoma effusions (25.4% of ovarian and 50% of breast carcinomas). However, PINCH-2 protein expression by flow cytometry did not differ significantly between the three cancer types. No association was observed between PINCH-2 levels and patient survival or expression of previously-studied molecules related to adhesion, metastasis and apoptosis inhibition in ovarian carcinoma.
conclusionsOur data suggest that PINCH-2 mRNA is overexpressed in malignant mesothelioma compared with carcinomas involving serosal cavities, and that low levels of this gene argue against the diagnosis of mesothelioma. The frequent PINCH-2 protein expression in all three studied cancers suggests a role for this molecule in cancer cell biology in effusions and merits further research.
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