ArticlePloS one2010
Sphingosine kinase-1 (SphK-1) regulates Mycobacterium smegmatis infection in macrophages.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 33 citations in OpenAlex.
- Host-strain compatibility shapes host transcriptional responses in macrophageMicrobial genomics · 2026Article
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- Signaling controversy and future therapeutical perspectives of targeting sphingolipid network in cancer immune editing and resistance to tumor necrosis factor-α immunotherapy.Cell communication and signaling : CCS · 2024Review
- The Impact of Sphingosine Kinases on Inflammation-Induced Cytokine Release and Vascular Endothelial Barrier Integrity.International journal of molecular sciences · 2022Article
- Sphingolipids, mycobacteria and host: Unraveling the tug of war.Frontiers in immunology · 2022Article
- Role of sphingosine kinase and sphingosine-1-phosphate receptor in the liver pathology of mice infected with Plasmodium berghei ANKA.PloS one · 2022Article
- Lipid larceny: channelizing host lipids for establishing successful pathogenesis by bacteria.Virulence · 2021Review
- PET Study of Sphingosine-1-phosphate Receptor 1 Expression in Response toMolecular imaging · 2021Article
- Sphingosine-1-Phosphate (S-1P) Promotes Differentiation of Naive Macrophages and Enhances Protective Immunity AgainstFrontiers in immunology · 2019Article
- Sphingosine-1-Phosphate and Macrophage Biology-How the Sphinx Tames the Big Eater.Frontiers in immunology · 2019Review
- A Comprehensive Review on the Manipulation of the Sphingolipid Pathway by Pathogenic Bacteria.Frontiers in cell and developmental biology · 2019Review
- Sphingosine-1-phosphate signaling in Leishmania donovani infection in macrophages.PLoS neglected tropical diseases · 2018Article
- Biglycan- and Sphingosine Kinase-1 Signaling Crosstalk Regulates the Synthesis of Macrophage Chemoattractants.International journal of molecular sciences · 2017Article
- Sphingolipids Are Dual Specific Drug Targets for the Management of Pulmonary Infections: Perspective.Frontiers in immunology · 2017Review
- Elucidating population-wide mycobacterial replication dynamics at the single-cell level.Microbiology (Reading, England) · 2016Article
- Activation of ATP secretion via volume-regulated anion channels by sphingosine-1-phosphate in RAW macrophages.Pflugers Archiv : European journal of physiology · 2015Article
- Sphingosine kinases are not required for inflammatory responses in macrophages.The Journal of biological chemistry · 2013Article
- Sphingosine-1-phosphate, FTY720, and sphingosine-1-phosphate receptors in the pathobiology of acute lung injury.American journal of respiratory cell and molecular biology · 2013Review
- Sphingosine 1-phosphate protects primary human keratinocytes from apoptosis via nitric oxide formation through the receptor subtype S1P₃.Molecular and cellular biochemistry · 2012Article
- The role of sphingosine-1-phosphate transporter Spns2 in immune system function.Journal of immunology (Baltimore, Md. : 1950) · 2012Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sphingosine kinase-1 is known to mediate Mycobacterium smegmatis induced inflammatory responses in macrophages, but its role in controlling infection has not been reported to date. We aimed to unravel the significance of SphK-1 in controlling M. smegmatis infection in RAW 264.7 macrophages. Our results demonstrated for the first time that selective inhibition of SphK-1 by either D, L threo dihydrosphingosine (DHS; a competitive inhibitor of Sphk-1) or Sphk-1 siRNA rendered RAW macrophages sensitive to M. smegmatis infection. This was due to the reduction in the expression of iNOs, p38, pp-38, late phagosomal marker, LAMP-2 and stabilization of the RelA (pp-65) subunit of NF-kappaB. This led to a reduction in the generation of NO and secretion of TNF-alpha in infected macrophages. Congruently, overexpression of SphK-1 conferred resistance in macrophages to infection which was due to enhancement in the generation of NO and expression of iNOs, pp38 and LAMP-2. In addition, our results also unraveled a novel regulation of p38MAPK by SphK-1 during M. smegmatis infection and generation of NO in macrophages. Enhanced NO generation and expression of iNOs in SphK-1++ infected macrophages demonstrated their M-1(bright) phenotype of these macrophages. These findings thus suggested a novel antimycobacterial role of SphK-1 in macrophages.
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