ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2010
Tissue and cellular expression patterns of porcine CFTR: similarities to and differences from human CFTR.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- Article
- The molecular evolution of function in the CFTR chloride channel.The Journal of general physiology · 2021Article
- Cystic fibrosis transmembrane conductance regulator-emerging regulator of cancer.Cellular and molecular life sciences : CMLS · 2018Review
- A sequence upstream of canonical PDZ-binding motif within CFTR COOH-terminus enhances NHERF1 interaction.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Ubiquitous LEA29Y Expression Blocks T Cell Co-Stimulation but Permits Sexual Reproduction in Genetically Modified Pigs.PloS one · 2016Article
- Naturally Occurring Deletion Mutants of the Pig-Specific, Intestinal Crypt Epithelial Cell Protein CLCA4b without Apparent Phenotype.PloS one · 2015Article
- Regulation of ACh release from guinea pig bladder urothelial cells: potential role in bladder filling sensations.British journal of pharmacology · 2014Article
- Efficient gene delivery to pig airway epithelia and submucosal glands using helper-dependent adenoviral vectors.Molecular therapy. Nucleic acids · 2013Article
- Airway deposition of nebulized gene delivery nanocomplexes monitored by radioimaging agents.American journal of respiratory cell and molecular biology · 2013Article
- Sequential targeting of CFTR by BAC vectors generates a novel pig model of cystic fibrosis.Journal of molecular medicine (Berlin, Germany) · 2012Article
- The porcine chloride channel calcium-activated family member pCLCA4a mirrors lung expression of the human hCLCA4.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2012Article
- The murine goblet cell protein mCLCA3 is a zinc-dependent metalloprotease with autoproteolytic activity.Molecules and cells · 2011Article
- The Chloride Channel Regulator, Calcium-Activated-1 Is Expressed in Synoviocytes and Articular Chondrocytes in Health and Disease.The journal of histochemistry and cytochemistry : official journal of the Histochemistry SocietyArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Emerging porcine models of cystic fibrosis (CF) are expected to mimic the human disease more closely than current mouse models do. However, little is known of the tissue and cellular expression patterns of the porcine CF transmembrane conductance regulator (pCFTR) and possible differences from human CFTR (hCFTR). Here, the expression pattern of pCFTR was systematically established on the mRNA and protein levels. Using specific anti-pCFTR antibodies, the majority of the protein was immunohistochemically detected on paraffin-embedded sections and on cryostate sections in the apical cytosol of intestinal crypt epithelial cells, nasal, tracheal, and bronchial epithelial cells, and other select, mostly glandular epithelial cells. Confocal laser scanning microscopy with co-localization of the Golgi marker 58K localized the protein in the cytosol between the Golgi apparatus and the apical cell membrane with occasional punctate or diffuse staining of the apical membrane. The tissue and cellular distribution patterns were confirmed by RT-PCR from whole tissue lysates or select cells after laser capture microdissection. Thus, expression of pCFTR was found to largely resemble that of hCFTR except for the kidney, brain, and cutaneous glands, which lack expression in pigs. Species-specific differences between pCFTR and hCFTR may become relevant for future interpretations of the CF phenotype in pig models.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.