Evidence map›Paper›PMID 20498320›Full record

ArticleAntimicrobial agents and chemotherapy2010

High-resolution melt curve analysis for identification of single nucleotide mutations in the quinolone resistance gene aac(6')-Ib-cr.

Carlos Hidalgo-Grass, Jacob Strahilevitz

Abstract readEvaluation Study
In one paragraph

Article in Antimicrobial agents and chemotherapy, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. First Report ofInternational journal of molecular sciences · 2023
    Article
  4. Clinically RelevantMicroorganisms · 2021
    Article
  5. Article
  6. Article
  7. Rapid detection of AAC(6')-Ib-cr production using a MALDI-TOF MS strategy.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2016
    Article
  8. Article
  9. Plasmid-mediated quinolone resistance.Microbiology spectrum · 2014
    Review
  10. Article
  11. Article
  12. Aminoglycoside modifying enzymes.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2010
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Carlos Hidalgo-GrassDepartment of Clinical Microbiology and Infectious Diseases, Hadassah Ein-Kerem, Jerusalem 91120, Israel. carloshg@hadassah.org.il
Jacob Strahilevitz

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We have developed a simple PCR-based high-resolution melt curve analysis for identification of the quinolone resistance gene aac(6')-Ib-cr through regions encompassing the two defining single nucleotide mutations. Dissociation curves showed 100% concordance with DNA sequencing, including the identification of a strain where aac(6')-Ib and aac(6')-Ib-cr coexist.

Indexed as

MutationTransition TemperatureAcetyltransferasesAnti-Bacterial AgentsDrug Resistance, BacterialEnterobacteriaceaeEnterobacteriaceae InfectionsHumansMicrobial Sensitivity TestsPolymerase Chain ReactionQuinolonesAcetyltransferasesaminoglycoside acetyltransferaseAnti-Bacterial AgentsQuinolones

Identifiers

PMID20498320
PMCPMC2916293

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.