ArticleGenetics2010
Parallel genetic and proteomic screens identify Msps as a CLASP-Abl pathway interactor in Drosophila.
Article in Genetics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 33 citations in OpenAlex.
- Abl2 repairs microtubules and phase separates with tubulin to promote microtubule nucleation.Current biology : CB · 2023Article
- Control of Cell Growth and Proliferation by the Tribbles Pseudokinase: Lessons from Drosophila.Cancers · 2021Review
- Investigating the impact of the phosphorylation status of tyrosine residues within the TACC domain of TACC3 on microtubule behavior during axon growth and guidance.Cytoskeleton (Hoboken, N.J.) · 2020Article
- Regulation of MT dynamics via direct binding of an Abl family kinase.The Journal of cell biology · 2019Article
- XMAP215 promotes microtubule-F-actin interactions to regulate growth cone microtubules during axon guidance inJournal of cell science · 2019Article
- Distinct Functions for Mammalian CLASP1 and -2 During Neurite and Axon Elongation.Frontiers in cellular neuroscience · 2019Article
- Article
- The microtubule plus-end-tracking protein TACC3 promotes persistent axon outgrowth and mediates responses to axon guidance signals during development.Neural development · 2017Article
- Cytoskeletal social networking in the growth cone: How +TIPs mediate microtubule-actin cross-linking to drive axon outgrowth and guidance.Cytoskeleton (Hoboken, N.J.) · 2016Review
- Quantitative Assessment of Eye Phenotypes for Functional Genetic Studies Using Drosophila melanogaster.G3 (Bethesda, Md.) · 2016Article
- Drosophila KASH-domain protein Klarsicht regulates microtubule stability and integrin receptor localization during collective cell migration.Developmental biology · 2015Article
- Drosophila CLIP-190 and mammalian CLIP-170 display reduced microtubule plus end association in the nervous system.Molecular biology of the cell · 2015Article
- TOG Proteins Are Spatially Regulated by Rac-GSK3β to Control Interphase Microtubule Dynamics.PloS one · 2015Article
- TIPsy tour guides: how microtubule plus-end tracking proteins (+TIPs) facilitate axon guidance.Frontiers in cellular neuroscience · 2015Review
- The Abl/enabled signaling pathway regulates Golgi architecture in Drosophila photoreceptor neurons.Molecular biology of the cell · 2014Article
- Abelson phosphorylation of CLASP2 modulates its association with microtubules and actin.Cytoskeleton (Hoboken, N.J.) · 2014Article
- Orbit/CLASP is required for myosin accumulation at the cleavage furrow in Drosophila male meiosis.PloS one · 2014Article
- Growth cone-specific functions of XMAP215 in restricting microtubule dynamics and promoting axonal outgrowth.Neural development · 2013Article
- Host microtubule plus-end binding protein CLASP1 influences sequential steps in the Trypanosoma cruzi infection process.Cellular microbiology · 2013Article
- Multiparametric analysis of CLASP-interacting protein functions during interphase microtubule dynamics.Molecular and cellular biology · 2013Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
Regulation of cytoskeletal structure and dynamics is essential for multiple aspects of cellular behavior, yet there is much to learn about the molecular machinery underlying the coordination between the cytoskeleton and its effector systems. One group of proteins that regulate microtubule behavior and its interaction with other cellular components, such as actin-regulatory proteins and transport machinery, is the plus-end tracking proteins (MT+TIPs). In particular, evidence suggests that the MT+TIP, CLASP, may play a pivotal role in the coordination of microtubules with other cellular structures in multiple contexts, although the molecular mechanism by which it functions is still largely unknown. To gain deeper insight into the functional partners of CLASP, we conducted parallel genetic and proteome-wide screens for CLASP interactors in Drosophila melanogaster. We identified 36 genetic modifiers and 179 candidate physical interactors, including 13 that were identified in both data sets. Grouping interactors according to functional classifications revealed several categories, including cytoskeletal components, signaling proteins, and translation/RNA regulators. We focused our initial investigation on the MT+TIP Minispindles (Msps), identified among the cytoskeletal effectors in both genetic and proteomic screens. Here, we report that Msps is a strong modifier of CLASP and Abl in the retina. Moreover, we show that Msps functions during axon guidance and antagonizes both CLASP and Abl activity. Our data suggest a model in which CLASP and Msps converge in an antagonistic balance in the Abl signaling pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.