ArticleMolecular and cellular biology2010
RNA polymerase II C-terminal domain phosphorylation patterns in Caenorhabditis elegans operons, polycistronic gene clusters with only one promoter.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Multi-gene Co-expression systems inSynthetic and systems biotechnology · 2026Review
- DAF-16/FOXO requires Protein Phosphatase 4 to initiate transcription of stress resistance and longevity promoting genes.Nature communications · 2020Article
- The Caenorhabditis elegans Ortholog of TDP-43 Regulates the Chromatin Localization of the Heterochromatin Protein 1 Homolog HPL-2.Molecular and cellular biology · 2018Article
- Histone H3K9 and H4 Acetylations and Transcription Facilitate the Initial CENP-AEpigenetics & chromatin · 2018Article
- Localization of RNAPII and 3' end formation factor CstF subunits on C. elegans genes and operons.Transcription · 2016Article
- TDP-1, the Caenorhabditis elegans ortholog of TDP-43, limits the accumulation of double-stranded RNA.The EMBO journal · 2014Article
- Coupling mRNA processing with transcription in time and space.Nature reviews. Genetics · 2014Review
- 3' end formation of pre-mRNA and phosphorylation of Ser2 on the RNA polymerase II CTD are reciprocally coupled in human cells.Genes & development · 2014Article
- Inhibition of post-transcriptional RNA processing by CDK inhibitors and its implication in anti-viral therapy.PloS one · 2014Article
- Transcriptional regulation of gene expression in C. elegans.WormBook : the online review of C. elegans biology · 2013Review
- RSR-2, the Caenorhabditis elegans ortholog of human spliceosomal component SRm300/SRRM2, regulates development by influencing the transcriptional machinery.PLoS genetics · 2013Article
- RNA polymerase II transcription elongation and Pol II CTD Ser2 phosphorylation: A tail of two kinases.Nucleus (Austin, Tex.)Review
- CCDC-55 is required for larval development and distal tip cell migration in Caenorhabditis elegans.Mechanisms of developmentArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The heptad repeat of the RNA polymerase II (RNAPII) C-terminal domain is phosphorylated at serine 5 near gene 5' ends and serine 2 near 3' ends in order to recruit pre-mRNA processing factors. Ser-5(P) is associated with gene 5' ends to recruit capping enzymes, whereas Ser-2(P) is associated with gene 3' ends to recruit cleavage and polyadenylation factors. In the gene clusters called operons in Caenorhabditis elegans, there is generally only a single promoter, but each gene in the operon forms a 3' end by the usual mechanism. Although downstream operon genes have 5' ends, they receive their caps by trans splicing rather than by capping enzymes. Thus, they are predicted to not need Ser-5 phosphorylation. Here we show by RNAPII chromatin immunoprecipitation (ChIP) that internal operon gene 5' ends do indeed lack Ser-5(P) peaks. In contrast, Ser-2(P) peaks occur at each mRNA 3' end, where the 3'-end formation machinery binds. These results provide additional support for the idea that the serine phosphorylation of the C-terminal domain (CTD) serves to bring RNA-processing enzymes to the transcription complex. Furthermore, these results provide a novel demonstration that genes in operons are cotranscribed from a single upstream promoter.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.