ArticleMolecular and cellular biology2010
HuR uses AUF1 as a cofactor to promote p16INK4 mRNA decay.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
68 citing papers in PubMed, 111 citations in OpenAlex.
- RNA binding proteins as mediators of pathological cardiac remodeling.Frontiers in cell and developmental biology · 2024Review
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- Seeking a Role for Translational Control by Alternative Polyadenylation inMicroorganisms · 2021Review
- HuB and HuD repress telomerase activity by dissociating HuR from TERC.Nucleic acids research · 2021Article
- Long Noncoding RNA OIP5-AS1 Promotes Cell Apoptosis and Cataract Formation by Blocking POLG Expression Under Oxidative StressInvestigative ophthalmology & visual science · 2020Article
- MicroRNA-Independent Modulation of DICER1 Expression by hAgo2.Molecular and cellular biology · 2020Article
- Review
- Regulation of senescence traits by MAPKs.GeroScience · 2020Review
- Characterization of novel LncRNA P14AS as a protector of ANRIL through AUF1 binding in human cells.Molecular cancer · 2020Article
- tRNA-Derived Small RNAs and Their Potential Roles in Cardiac Hypertrophy.Frontiers in pharmacology · 2020Review
- Post-transcriptional regulation of Nrf2-mRNA by the mRNA-binding proteins HuR and AUF1.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Circular RNA circAGO2 drives cancer progression through facilitating HuR-repressed functions of AGO2-miRNA complexes.Cell death and differentiation · 2019Article
- The RGG/RG motif of AUF1 isoform p45 is a key modulator of the protein's RNA chaperone and RNA annealing activities.RNA biology · 2019Article
- LncRNAs Regulatory Networks in Cellular Senescence.International journal of molecular sciences · 2019Review
- Blockage of glutamine-dependent anaplerosis affects mTORC1/2 activity and ultimately leads to cellular senescence-like response.Biology open · 2019Article
- MIR100 host gene-encoded lncRNAs regulate cell cycle by modulating the interaction between HuR and its target mRNAs.Nucleic acids research · 2018Article
- Article
- Post-transcriptional regulation of Pabpn1 by the RNA binding protein HuR.Nucleic acids research · 2018Article
- Loss of RNA-binding protein HuR facilitates cellular senescence through posttranscriptional regulation of TIN2 mRNA.Nucleic acids research · 2018Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
In this study, we show that HuR destabilizes p16(INK4) mRNA. Although the knockdown of HuR or AUF1 increased p16 expression, concomitant AUF1 and HuR knockdown had a much weaker effect. The knockdown of Ago2, a component of the RNA-induced silencing complex (RISC), stabilized p16 mRNA. The knockdown of HuR diminished the association of the p16 3' untranslated region (3'UTR) with AUF1 and vice versa. While the knockdown of HuR or AUF1 reduced the association of Ago2 with the p16 3'UTR, Ago2 knockdown had no influence on HuR or AUF1 binding to the p16 3'UTR. The use of EGFP-p16 chimeric reporter transcripts revealed that p16 mRNA decay depended on a stem-loop structure present in the p16 3'UTR, as HuR and AUF1 destabilized EGFP-derived chimeric transcripts bearing wild-type sequences but not transcripts with mutations in the stem-loop structure. In senescent and HuR-silenced IDH4 human diploid fibroblasts, the EGFP-p16 3'UTR transcript was more stable. Our results suggest that HuR destabilizes p16 mRNA by recruiting the RISC, an effect that depends on the secondary structure of the p16 3'UTR and requires AUF1 as a cofactor.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.