ArticleMolecular and cellular biology2010
Interplay between DNA methylation and transcription factor availability: implications for developmental activation of the mouse Myogenin gene.
Article in Molecular and cellular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 89 citations in OpenAlex.
- DNA methylation patterns are influenced by Pax3::Foxo1 expression and developmental lineage in rhabdomyosarcoma tumours forming in genetically engineered mouse models.The Journal of pathology · 2025Article
- Rockfish: A transformer-based model for accurate 5-methylcytosine prediction from nanopore sequencing.Nature communications · 2024Article
- AnAnimals : an open access journal from MDPI · 2022Article
- Muscle regeneration controlled by a designated DNA dioxygenase.Cell death & disease · 2021Article
- Epigenetic regulation of satellite cell fate during skeletal muscle regeneration.Skeletal muscle · 2021Review
- Global DNA methylation pattern involved in the modulation of differentiation potential of adipogenic and myogenic precursors in skeletal muscle of pigs.Stem cell research & therapy · 2020Article
- Mitochondrial Function in Muscle Stem Cell Fates.Frontiers in cell and developmental biology · 2020Review
- Expression Characteristics of Genes Hypermethylated and Downregulated in Rat Liver Specific to Nongenotoxic Hepatocarcinogens.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- DNMT1 inJournal of the American Society of Nephrology : JASN · 2019Article
- Ten-Eleven Translocation-2 (Tet2) Is Involved in Myogenic Differentiation of Skeletal Myoblast Cells in Vitro.Scientific reports · 2017Article
- Article
- Refinement of the androgen response element based on ChIP-Seq in androgen-insensitive and androgen-responsive prostate cancer cell lines.Scientific reports · 2016Article
- Transcriptional regulation of the potential tumor suppressor ABI3 gene in thyroid carcinomas: interplay between methylation and NKX2-1 availability.Oncotarget · 2016Article
- DNA Methylation in Skeletal Muscle Stem Cell Specification, Proliferation, and Differentiation.Stem cells international · 2016Review
- Regulation of Muscle Stem Cell Functions: A Focus on the p38 MAPK Signaling Pathway.Frontiers in cell and developmental biology · 2016Review
- DNA Methylation Dynamics During Differentiation, Proliferation, and Tumorigenesis in the Intestinal Tract.Stem cells and development · 2015Review
- Article
- Tissue-specific epigenetics in gene neighborhoods: myogenic transcription factor genes.Human molecular genetics · 2015Article
- Epigenetic Modification of Cloned Embryos Improves Nanog Reprogramming in Pigs.Cellular reprogramming · 2015Article
- Modeling the relationship of epigenetic modifications to transcription factor binding.Nucleic acids research · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During development, gene activation is stringently regulated to restrict expression only to the correct cell type and correct developmental stage. Here, we present mechanistic evidence that suggests DNA methylation contributes to this regulation by suppressing premature gene activation. Using the mouse Myogenin promoter as an example of the weak CpG island class of promoters, we find that it is initially methylated but becomes demethylated as development proceeds. Full hypersensitive site formation of the Myogenin promoter requires both the MEF2 and SIX binding sites, but binding to only one site can trigger the partial chromatin opening of the nonmethylated promoter. DNA methylation markedly decreases hypersensitive site formation that now occurs at a detectable level only when binding to both MEF2 and SIX binding sites is possible. This suggests that the probability of activating the methylated promoter is low until two of the factors are coexpressed within the same cell. Consistent with this, the single-cell analysis of developing somites shows that the coexpression of MEF2A and SIX1, which bind the MEF2 and SIX sites, correlates with the fraction of cells that demethylate the Myogenin promoter. Taken together, these studies imply that DNA methylation helps to prevent inappropriate gene activation until sufficient activating factors are coexpressed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.