ArticleBMC urology2010
Removal of urothelium affects bladder contractility and release of ATP but not release of NO in rat urinary bladder.
Article in BMC urology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 43 citations in OpenAlex.
- The Antipsychotic Aripiprazole Induces Cytotoxicity in Bladder Cancer Cells While Preserving Urothelial and Bladder Function.Pharmacology research & perspectives · 2026Article
- Intravesical substance P enhances bladder afferent nerve activity without the influence of the micturition reflex.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Prostaglandin E2 modulates contractility of mouse bladder smooth muscle cells.Frontiers in physiology · 2026Article
- Article
- Review
- Urothelium removal does not impact mucosal activity in response to muscarinic or adrenergic receptor stimulation.Tissue barriers · 2023Article
- The nitric oxide-cyclic guanosine monophosphate pathway inhibits the bladder ATP release in response to a physiological or pathological stimulus.Physiological reports · 2021Article
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Voiding Dysfunction in Old Male Rats Associated With Enlarged Prostate and Irregular Afferent-Triggered Reflex Responses.International neurourology journal · 2020Article
- Modulatory effects of intravesical P2X2/3 purinergic receptor inhibition on lower urinary tract electromyographic properties and voiding function of female rats with moderate or severe spinal cord injury.BJU international · 2019Article
- Modulation of lower urinary tract smooth muscle contraction and relaxation by the urothelium.Naunyn-Schmiedeberg's archives of pharmacology · 2018Review
- Octodon degus, a new model to study the agonist and plexus-induced response in the urinary bladder.Journal of physiology and biochemistry · 2017Article
- Fiber type-specific afferent nerve activity induced by transient contractions of rat bladder smooth muscle in pathological states.PloS one · 2017Article
- The Role of the Mucosa in Normal and Abnormal Bladder Function.Basic & clinical pharmacology & toxicology · 2016Review
- A1 Adenosine Receptor-Mediated Inhibition of Parasympathetic Neuromuscular Transmission in Human and Murine Urinary Bladder.The Journal of pharmacology and experimental therapeutics · 2016Article
- Receptors involved in the modulation of guinea pig urinary bladder motility by prostaglandin D2.British journal of pharmacology · 2015Article
- NTPDase3 and ecto-5'-nucleotidase/CD73 are differentially expressed during mouse bladder cancer progression.Purinergic signalling · 2014Article
- Signalling molecules in the urothelium.BioMed research international · 2014Review
- Cascade bioassay evidence for the existence of urothelium-derived inhibitory factor in Guinea pig urinary bladder.PloS one · 2014Article
- Diabetic plasticity of non-adrenergic non-cholinergic and P2X-mediated rat bladder contractions.Brain research bulletin · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe objective of our work was to investigate both the contractile function and the release of ATP and NO from strips of bladder tissue after removal of the urothelium.
methodsThe method of removal was a gentle swabbing motion rather than a sharp surgical cutting to separate the urothelium from the smooth muscle. The contractile response and ATP and NO release were measured in intact as well as on swabbed preparations. The removal of the urothelial layer was affirmed microscopically.
resultsAfter the swabbing, the smaller contractions were evoked by electrical as well as by chemical stimulation (50 microM carbachol or 50 microM alpha, beta meATP). Electrical stimulation, carbachol and substance P (5 microM) evoked lower release of ATP in the swabbed strips than in intact strips. Although release of NO evoked by electrical stimulation or substance P was not changed, release of NO evoked by carbachol was significantly less in the swabbed preparations.
conclusionSince swabbing removes only the urothelium, the presence of the suburothelial layer may explain the difference between our findings and those of others who found an increase in contractility. Evoked release of ATP is reduced in swabbed strips, indicating that ATP derives solely from the urothelium. On the other hand, electrical stimulation and substance P evoke identical degrees of NO release in both intact and swabbed preparations, suggesting that NO can be released from the suburothelium. Conversely, carbachol-induced release of NO is lower in swabbed strips, implying that the cholinergic receptors (muscarinic or nicotinic) are located in the upper layer of the urothelium.
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Registered trials
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