ArticleThe Plant journal : for cell and molecular biology2010
The small heat shock protein 20 RSI2 interacts with and is required for stability and function of tomato resistance protein I-2.
Article in The Plant journal : for cell and molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 84 citations in OpenAlex.
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- HSP104 and HSP20-L Are Required by Aspergillus nidulans in Response to Attack by Fungivorous Springtail Sinella curviseta.Environmental microbiology reports · 2025Article
- TaHSP18.6 and TaSRT1 interact to confer resistance toProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Multiplexed Host-Induced Gene Silencing ofToxins · 2023Article
- Histological and molecular responses of Vigna angularis to Uromyces vignae infection.BMC plant biology · 2022Article
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- Deciphering the Crosstalk Mechanisms of Wheat-Stem Rust Pathosystem: Genome-Scale Prediction Unravels Novel Host Targets.Frontiers in plant science · 2022Article
- Fine mapping a quantitative trait locus underlying seedling resistance to gummy stem blight using a residual heterozygous lines-derived strategy in cucumber.Frontiers in plant science · 2022Article
- SLI1 confers broad-spectrum resistance to phloem-feeding insects.Plant, cell & environment · 2021Article
- Proteomic analysis of early-stage incompatible and compatible interactions between grapevine and P. viticola.Horticulture research · 2021Article
- Haplotype- and SNP-Based GWAS for Growth and Wood Quality Traits inPlants (Basel, Switzerland) · 2021Article
- The soybean gene GmHsp22.4 is involved in the resistance response to Meloidogyne javanica in Arabidopsis thaliana.BMC plant biology · 2020Article
- Transcriptomic profiling of Solanum peruvianum LA3858 revealed a Mi-3-mediated hypersensitive response to Meloidogyne incognita.BMC genomics · 2020Article
- A Sec-Dependent Secretory Protein of the Huanglongbing-Associated Pathogen Suppresses Hypersensitive Cell Death inFrontiers in microbiology · 2020Article
- Heat Shock Proteins: Dynamic Biomolecules to Counter Plant Biotic and Abiotic Stresses.International journal of molecular sciences · 2019Review
- TheInternational journal of molecular sciences · 2019Article
- Genome-Wide Analysis of Watermelon HSP20s and Their Expression Profiles and Subcellular Locations under Stresses.International journal of molecular sciences · 2018Article
- Full-Length Transcriptome Survey and Expression Analysis ofInternational journal of molecular sciences · 2018Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Race-specific disease resistance in plants depends on the presence of resistance (R) genes. Most R genes encode NB-ARC-LRR proteins that carry a C-terminal leucine-rich repeat (LRR). Of the few proteins found to interact with the LRR domain, most have proposed (co)chaperone activity. Here, we report the identification of RSI2 (Required for Stability of I-2) as a protein that interacts with the LRR domain of the tomato R protein I-2. RSI2 belongs to the family of small heat shock proteins (sHSPs or HSP20s). HSP20s are ATP-independent chaperones that form oligomeric complexes with client proteins to prevent unfolding and subsequent aggregation. Silencing of RSI2-related HSP20s in Nicotiana benthamiana compromised the hypersensitive response that is normally induced by auto-active variants of I-2 and Mi-1, a second tomato R protein. As many HSP20s have chaperone properties, the involvement of RSI2 and other R protein (co)chaperones in I-2 and Mi-1 protein stability was examined. RSI2 silencing compromised the accumulation of full-length I-2 in planta, but did not affect Mi-1 levels. Silencing of heat shock protein 90 (HSP90) and SGT1 led to an almost complete loss of full-length I-2 accumulation and a reduction in Mi-1 protein levels. In contrast to SGT1 and HSP90, RSI2 silencing led to accumulation of I-2 breakdown products. This difference suggests that RSI2 and HSP90/SGT1 chaperone the I-2 protein using different molecular mechanisms. We conclude that I-2 protein function requires RSI2, either through direct interaction with, and stabilization of I-2 protein or by affecting signalling components involved in initiation of the hypersensitive response.
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