ArticleMolecular microbiology2010
A remote CheZ orthologue retains phosphatase function.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 26 citations in OpenAlex.
- Altering chemotaxis as a strategy to enhance the foraging range of motility-restricted bacteria.Communications biology · 2026Article
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- Article
- Analysis of CheW-like domains provides insights into organization of prokaryotic chemotaxis systems.Proteins · 2023Article
- The evolutionary path of chemosensory and flagellar macromolecular machines in Campylobacterota.PLoS genetics · 2022Article
- Azorhizobium caulinodans Chemotaxis Is Controlled by an Unusual Phosphorelay Network.Journal of bacteriology · 2022Article
- The Hypoxia-Associated Localization of Chemotaxis Protein CheZ inFrontiers in microbiology · 2021Article
- Protein Residues and a Novel Motif Involved in the Cellular Localization of CheZ inFrontiers in microbiology · 2020Article
- AApplied and environmental microbiology · 2018Article
- Colonization, localization, and inflammation: the roles of H. pylori chemotaxis in vivo.Current opinion in microbiology · 2018Review
- Cooperation of two distinct coupling proteins creates chemosensory network connections.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Helicobacter pylori CZB Cytoplasmic Chemoreceptor TlpD Forms an Autonomous Polar Chemotaxis Signaling Complex That Mediates a Tactic Response to Oxidative Stress.Journal of bacteriology · 2016Article
- Helicobacter pylori CheZ(HP) and ChePep form a novel chemotaxis-regulatory complex distinct from the core chemotaxis signaling proteins and the flagellar motor.Molecular microbiology · 2015Article
- A supplemented soft agar chemotaxis assay demonstrates the Helicobacter pylori chemotactic response to zinc and nickel.Microbiology (Reading, England) · 2013Article
- A Single-cell genome for Thiovulum sp.Applied and environmental microbiology · 2012Article
- Chemotaxis in Campylobacter jejuni.European journal of microbiology & immunology · 2012Review
- Sinorhizobium meliloti CheA complexed with CheS exhibits enhanced binding to CheY1, resulting in accelerated CheY1 dephosphorylation.Journal of bacteriology · 2012Article
- Motility and chemotaxis in Campylobacter and Helicobacter .Annual review of microbiology · 2011Review
- Article
- Conserved mechanism for sensor phosphatase control of two-component signaling revealed in the nitrate sensor NarX.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Aspartyl-phosphate phosphatases underlie the rapid responses of bacterial chemotaxis. One such phosphatase, CheZ, was originally proposed to be restricted to beta and gamma proteobacter, suggesting only a small subset of microbes relied on this protein. A putative CheZ phosphatase was identified genetically in the epsilon proteobacter Helicobacter pylori (Mol Micro 61:187). H. pylori utilizes a chemotaxis system consisting of CheAY, three CheVs, CheW, CheY(HP) and the putative CheZ to colonize the host stomach. Here we investigate whether this CheZ has phosphatase activity. We phosphorylated potential targets in vitro using either a phosphodonor or the CheAY kinase and [gamma-(32)P]-ATP, and found that H. pylori CheZ (CheZ(HP)) efficiently dephosphorylates CheY(HP) and CheAY and has additional weak activity on CheV2. We detected no phosphatase activity towards CheV1 or CheV3. Mutations corresponding to Escherichia coli CheZ active site residues or deletion of the C-terminal region inactivate CheZ(HP) phosphatase activity, suggesting the two CheZs function similarly. Bioinformatics analysis suggests that CheZ phosphatases are found in all proteobacteria classes, as well as classes Aquificae, Deferribacteres, Nitrospira and Sphingobacteria, demonstrating that CheZ phosphatases are broadly distributed within Gram-negative bacteria.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.