ArticleMolecular microbiology2010
RNA polymerase mutations that facilitate replication progression in the rep uvrD recF mutant lacking two accessory replicative helicases.
Article in Molecular microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- Interaction with single-stranded DNA-binding protein localizes ribonuclease HI to DNA replication forks and facilitates R-loop removal.Molecular microbiology · 2020Article
- Too Much of a Good Thing: How Ectopic DNA Replication Affects Bacterial Replication Dynamics.Frontiers in microbiology · 2020Review
- Article
- DksA and DNA double-strand break repair.Current genetics · 2019Review
- Article
- Direct removal of RNA polymerase barriers to replication by accessory replicative helicases.Nucleic acids research · 2019Article
- DksA-RNA polymerase interactions support new origin formation and DNA repair in Escherichia coli.Molecular microbiology · 2019Article
- The Clash of Macromolecular Titans: Replication-Transcription Conflicts in Bacteria.Annual review of microbiology · 2018Review
- Replication Fork Breakage and Restart in Escherichia coli.Microbiology and molecular biology reviews : MMBR · 2018Review
- Replication Restart in Bacteria.Journal of bacteriology · 2017Review
- Inhibiting translation elongation can aid genome duplication in Escherichia coli.Nucleic acids research · 2017Article
- Article
- Transcription-replication conflicts: how they occur and how they are resolved.Nature reviews. Molecular cell biology · 2016Review
- Article
- Mutations in the CRE pocket of bacterial RNA polymerase affect multiple steps of transcription.Nucleic acids research · 2015Article
- Interaction with Single-stranded DNA-binding Protein Stimulates Escherichia coli Ribonuclease HI Enzymatic Activity.The Journal of biological chemistry · 2015Article
- The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units.PLoS genetics · 2015Article
- Acid-adapted strains of Escherichia coli K-12 obtained by experimental evolution.Applied and environmental microbiology · 2015Article
- RNA polymerase II contributes to preventing transcription-mediated replication fork stalls.The EMBO journal · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We observed that cells lacking Rep and UvrD, two replication accessory helicases, and the recombination protein RecF are cryo-sensitive on rich medium. We isolated five mutations that suppress this Luria-Bertani (LB)-cryo-sensitivity and show that they map in the genes encoding the RNA polymerase subunits RpoB and RpoC. These rpoB (D444G, H447R and N518D) and rpoC mutants (H113R and P451L) were characterized. rpoB(H447R) and rpoB(D444G) prevent activation of the Prrn core promoter in rich medium, but only rpoB(H447R) also suppresses the auxotrophy of a relA spoT mutant (stringent-like phenotype). rpoC(H113R) suppresses the thermo-sensitivity of a greA greB mutant, suggesting that it destabilizes stalled elongation complexes. All mutations but rpoC(P451L) prevent R-loop formation. We propose that these rpo mutations allow replication in the absence of Rep and UvrD by destabilizing RNA Pol upon replication-transcription collisions. In a RecF(+) context, they improve growth of rep uvrD cells only if DinG is present, supporting the hypothesis that Rep, UvrD and DinG facilitate progression of the replication fork across transcribed sequences. They rescue rep uvrD dinG recF cells, indicating that in a recF mutant replication forks arrested by unstable transcription complexes can restart without any of the three known replication accessory helicases Rep, UvrD and DinG.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.