ArticleBiology of the cell2010
Munc18 and Munc13 regulate early neurite outgrowth.
Article in Biology of the cell, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Meta-analysis of pharmacogenetic interactions in amyotrophic lateral sclerosis clinical trials.Neurology · 2017Pooled it
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- An active vesicle priming machinery suppresses axon regeneration upon adult CNS injury.Neuron · 2022Article
- Presynaptic Short-Term Plasticity Persists in the Absence of PKC Phosphorylation of Munc18-1.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Adult Neural Plasticity in Naked Mole-Rats: Implications of Fossoriality, Longevity and Sociality on the Brain's Capacity for Change.Advances in experimental medicine and biology · 2021Article
- Entacapone Treatment Modulates Hippocampal Proteins Related to Synaptic Vehicle Trafficking.Cells · 2020Article
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- Beyond the cytoskeleton: The emerging role of organelles and membrane remodeling in the regulation of axon collateral branches.Developmental neurobiology · 2016Review
- Reaching Out to Send a Message: Proteins Associated with Neurite Outgrowth and Neurotransmission are Altered with Age in the Long-Lived Naked Mole-Rat.Neurochemical research · 2016Article
- Membrane Trafficking in Neuronal Development: Ins and Outs of Neural Connectivity.International review of cell and molecular biology · 2016Article
- Essential roles for the splicing regulator nSR100/SRRM4 during nervous system development.Genes & development · 2015Article
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- Intercellular protein-protein interactions at synapses.Protein & cell · 2014Review
- UNC13A influences survival in Italian amyotrophic lateral sclerosis patients: a population-based study.Neurobiology of aging · 2013Article
- Syntaxin binding protein 1 is not required for allergic inflammation via IgE-mediated mast cell activation.PloS one · 2013Article
- Insight into the role of Ca2+-binding protein 5 in vesicle exocytosis.Investigative ophthalmology & visual science · 2011Article
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Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background informationDuring development, growth cones of outgrowing neurons express proteins involved in vesicular secretion, such as SNARE (soluble N-ethylmaleimide-sensitive fusion protein-attachment protein receptor) proteins, Munc13 and Munc18. Vesicles are known to fuse in growth cones prior to synapse formation, which may contribute to outgrowth.
resultsWe tested this possibility in dissociated cell cultures and organotypic slice cultures of two release-deficient mice (Munc18-1 null and Munc13-1/2 double null). Both types of release-deficient neurons have a decreased outgrowth speed and therefore have a smaller total neurite length during early development [DIV1-4 (day in vitro 1-4)]. In addition, more filopodia per growth cone were observed in Munc18-1 null, but not WT (wild-type) or Munc13-1/2 double null neurons. The smaller total neurite length during early development was no longer observed after synaptogenesis (DIV14-23).
conclusionThese data suggest that the inability of vesicle fusion in the growth cone affects outgrowth during the initial phases when outgrowth speed is high, but not during/after synaptogenesis. Overall, the outgrowth speed is probably not rate-limiting during neuronal network formation, at least in vitro. In addition, Munc18, but not Munc13, regulates growth cone filopodia, potentially via its previously observed effect on filamentous actin.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.