ArticleFish physiology and biochemistry2010
Development and characterization of a new marine fish cell line from turbot (Scophthalmus maximus).
Article in Fish physiology and biochemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 52 citations in OpenAlex.
- Establishment and Characterization of Hepatocyte Line from Turbot (Scophthalmus maximus L.) and Its Application in the Study of Glucose Metabolism.Marine biotechnology (New York, N.Y.) · 2025Article
- Transcriptome Analysis of Transiently Reversible Cell Vacuolization Caused by Excessive Serum Concentration inBiology · 2024Article
- pparβ regulates lipid catabolism by mediating acox and cpt-1 genes in Scophthalmus maximus under heat stress.Fish physiology and biochemistry · 2024Article
- Megalocytivirus and Other Members of the FamilyViruses · 2023Review
- Derivation and characterization of new cell line from intestine of turbot (Scophthalmus maximus).In vitro cellular & developmental biology. Animal · 2023Article
- Assessing Fish Immunotoxicity by Means ofFrontiers in immunology · 2022Review
- Heat stress-induced HSP90 expression is dependent on ERK and HSF1 activation in turbot (Scophthalmus maximus) kidney cells.Cell stress & chaperones · 2021Article
- Development and characterization of a new DRCF cell line from Indian wild strain zebrafish Danio rerio (Hamilton 1822).Fish physiology and biochemistry · 2020Article
- Establishment and characterization of a fibroblast-like cell line from the muscle of turbot (Scophthalmus maximus L.).Fish physiology and biochemistry · 2019Article
- Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara.Fish physiology and biochemistry · 2018Article
- Two skin cell lines from wild-type and albino Japanese flounder (Paralichthys olivaceus): establishment, characterization, virus susceptibility, efficient transfection, and application to albinism study.Fish physiology and biochemistry · 2017Article
- Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale.Fish physiology and biochemistry · 2015Article
- Article
- Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis).Fish physiology and biochemistry · 2012Article
- Establishment and characterization of a testicular cell line from the half-smooth tongue sole, Cynoglossus semilaevis.International journal of biological sciences · 2011Article
- Development, characterization, conservation and storage of fish cell lines: a review.Fish physiology and biochemistry · 2011Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new marine fish cell line, TK, derived from turbot (Scophthalmus maximus) kidney, was established by the method of trypsin digestion and subcultured for more than 50 passages over a period of 300 days. The TK cells were maintained in Minimum Essential Medium Eagle (MEM) supplemented with HEPES, antibiotics, fetal bovine serum (FBS), 2-Mercaptoethanol (2-Me), and basic fibroblast growth factor (bFGF). The suitable growth temperature for TK cells was 24°C, and microscopically, TK cells were composed of fibroblast-like cells. Chromosome analysis revealed that the TK cell line has a normal diploid karyotype with 2n=44. Two fish viruses LCDV-C (lymphocystis disease virus from China) and TRBIV (turbot reddish body iridovirus) were used to determine the virus susceptibility of TK cell line. The TK cell line was found to be susceptible to TRBIV, and the infection was confirmed by cytopathic effect (CPE) and transmission electron microscopy, which detected the viral particles in the cytoplasm of virus-infected cells. Finally, significant green fluorescent signals were observed when the TK cells were transfected with pEGFP-N3 vector, indicating its potential utility for fish virus study and genetic manipulation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.