ArticleNature neuroscience2010
A genetically targeted optical sensor to monitor calcium signals in astrocyte processes.
Article in Nature neuroscience, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 133 papers.
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Who cites it
133 citing papers in PubMed, 245 citations in OpenAlex.
- Calcium Buffering in Astrocytes and Its Relevance for Experimental Data Interpretation and Computational Modeling.Journal of neurochemistry · 2026Review
- In vivo multimodal neurochemical interfaces for real-time decoding of brain circuit.Nature reviews. Neuroscience · 2026Review
- Article
- Review
- CaNature communications · 2025Article
- Astrocyte morphogenesis requires self-recognition.Nature · 2025Article
- Amyloid-β-induced alteration of fast and localized calcium elevations in cultured astrocytes.Scientific reports · 2025Article
- Norepinephrine signals through astrocytes to modulate synapses.Science (New York, N.Y.) · 2025Article
- The Duality of Astrocyte Neuromodulation: Astrocytes Sense Neuromodulators and Are Neuromodulators.Journal of neurochemistry · 2025Review
- Low-frequency rTMS Plays a Neuroprotective role in Pilocarpine-induced Status Epilepticus Rat Models Through the AMPAR GluA1-STIM-CaMolecular neurobiology · 2025Article
- Astrocyte regulation of behavioral outputs: the versatile roles of calcium.Frontiers in cellular neuroscience · 2025Review
- Establishment and Use of Primary Cultured Astrocytes from Alexander Disease Model Mice.International journal of molecular sciences · 2024Article
- Revisiting astrocytic calcium signaling in the brain.Fundamental research · 2024Review
- Astrocyte morphology.Trends in cell biology · 2024Review
- A PACAP-activated network for secretion requires coordination of CaMolecular biology of the cell · 2024Article
- Phospholipase C-ε defines a PACAP-stimulated pathway for secretion in the chromaffin cell.Journal of neuroendocrinology · 2023Article
- Article
- Astrocyte heterogeneity and interactions with local neural circuits.Essays in biochemistry · 2023Article
- PACAP and acetylcholine cause distinct Ca2+ signals and secretory responses in chromaffin cells.The Journal of general physiology · 2023Article
- Diverse but unique astrocytic phenotypes during embryonic stem cell differentiation, culturing and development.Communications biology · 2023Article
73 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Calcium signaling is studied as a potential form of astrocyte excitability that may control astrocyte involvement in synaptic and cerebrovascular regulation. Fundamental questions remain unanswered about astrocyte calcium signaling, as current methods can not resolve calcium in small volume compartments, such as near the cell membrane and in distal cell processes. We modified the genetically encoded calcium sensor GCaMP2 with a membrane-tethering domain, Lck, increasing the level of Lck-GCaMP2 near the plasma membrane tenfold as compared with conventional GCaMP2. Using Lck-GCaMP2 in rat hippocampal astrocyte-neuron cocultures, we measured near-membrane calcium signals that were evoked pharmacologically or by single action potential-mediated neurotransmitter release. Moreover, we identified highly localized and frequent spontaneous calcium signals in astrocyte somata and processes that conventional GCaMP2 failed to detect. Lck-GCaMP2 acts as a genetically targeted calcium sensor for monitoring calcium signals in previously inaccessible parts of astrocytes, including fine processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.