ArticleJournal of bacteriology2010
Regulation of high-affinity iron acquisition homologues in the tsetse fly symbiont Sodalis glossinidius.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Heme-induced genes facilitate endosymbiont (Sodalis glossinidius) colonization of the tsetse fly (Glossina morsitans) midgut.PLoS neglected tropical diseases · 2022Article
- The iron transporter Transferrin 1 mediates homeostasis of the endosymbiotic relationship betweenmicroLife · 2021Article
- Growing Ungrowable Bacteria: Overview and Perspectives on Insect Symbiont Culturability.Microbiology and molecular biology reviews : MMBR · 2020Review
- Bacterial Symbionts of Tsetse Flies: Relationships and Functional Interactions Between Tsetse Flies and Their Symbionts.Results and problems in cell differentiation · 2020Review
- Thermal stress responses of Sodalis glossinidius, an indigenous bacterial symbiont of hematophagous tsetse flies.PLoS neglected tropical diseases · 2019Article
- TonB-dependent heme iron acquisition in the tsetse fly symbiont Sodalis glossinidius.Applied and environmental microbiology · 2015Article
- Comparison of potato and asian citrus psyllid adult and nymph transcriptomes identified vector transcripts with potential involvement in circulative, propagative liberibacter transmission.Pathogens (Basel, Switzerland) · 2014Article
- Characterization of the achromobactin iron acquisition operon in Sodalis glossinidius.Applied and environmental microbiology · 2013Article
- Tsetse-Wolbachia symbiosis: comes of age and has great potential for pest and disease control.Journal of invertebrate pathology · 2013Review
- Iron homeostasis in theAdvances in botanical research · 2013Article
- Candidatus Sodalis melophagi sp. nov.: phylogenetically independent comparative model to the tsetse fly symbiont Sodalis glossinidius.PloS one · 2012Article
- The haem-uptake gene cluster in Vibrio fischeri is regulated by Fur and contributes to symbiotic colonization.Environmental microbiology · 2011Article
- Lambda red-mediated genetic modification of the insect endosymbiont Sodalis glossinidius.Applied and environmental microbiology · 2011Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Sodalis glossinidius is a facultative intracellular bacterium that is a secondary symbiont of the tsetse fly (Diptera: Glossinidae). Since studies with other facultative intracellular bacteria have shown that high-affinity iron acquisition genes are upregulated in vivo, we investigated the regulation of several Sodalis genes that encode putative iron acquisition systems. These genes, SG1538 (hemT) and SG1516 (sitA), are homologous to genes encoding periplasmic heme and iron/manganese transporters, respectively. hemT promoter- and sitA promoter-gfp fusions were constructed, and in both Escherichia coli and Sodalis backgrounds, expression levels of these fusions were higher when the bacteria were grown in iron-limiting media than when the bacteria were grown in iron-replete media. The Sodalis promoters were tested for iron regulation in an E. coli strain that lacks the fur gene, which encodes the iron-responsive transcriptional repressor Fur. Expression of the promoter-gfp fusions in the E. coli fur mutant was constitutively high in both iron-replete and iron-deplete media, and addition of either Shigella flexneri fur or Sodalis fur to a plasmid restored normal regulation. A Sodalis fur mutant was constructed by intron mutagenesis, and semiquantitative reverse transcription-PCR (RT-PCR) showed that iron repression of sitA expression was also abolished in this strain. In vivo expression analysis showed that hemT and sitA are expressed when Sodalis is within tsetse fly hosts, suggesting a biological role for these genes when Sodalis is within the tsetse fly.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.