Evidence map›Paper›PMID 20494973›Full record

ArticleNucleic acids research2010

Origin and quantification of circulating DNA in mice with human colorectal cancer xenografts.

Alain R Thierry, Florent Mouliere, Celine Gongora, Jeremy Ollier, Bruno Robert, Marc Ychou, Maguy Del Rio, Franck Molina

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 156 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
156citing papers in PubMed, 4 pooled it
3.0field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

156 citing papers in PubMed, 4 syntheses or guidelines pooled it, 289 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Pooled it
  4. Circulating Cell-Free DNA and Colorectal Cancer: A Systematic Review.International journal of molecular sciences · 2018
    Pooled it
  5. Trial
  6. Circulating DNA Demonstrates Convergent Evolution and Common Resistance Mechanisms during Treatment of Colorectal Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2017
    Trial
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  9. Noninvasive saliva-based EGFR gene mutation detection in patients with lung cancer.American journal of respiratory and critical care medicine · 2014
    Trial
  10. Article
  11. Review
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  20. Article

96 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Alain R ThierrySysdiag UMR3145 - CNRS/BIO-RAD, CAP DELTA 1682 Rue de la Valsière, 34184 Montpellier CEDEX 4, France. alain.thierry@sysdiag.cnrs.fr
Florent Mouliere
Celine Gongora
Jeremy Ollier
Bruno Robert
Marc Ychou
Maguy Del Rio
Franck Molina
Institut National de Recherche en Santé Publique · MLModélisation et Ingénierie des Systèmes Complexes Biologiques pour le Diagnostic · FRCentre National de la Recherche Scientifique · FRInstitut de Recherche en Cancérologie de Montpellier · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although circulating DNA (ctDNA) could be an attractive tool for early cancer detection, diagnosis, prognosis, monitoring or prediction of response to therapies, knowledge on its origin, form and rate of release is poor and often contradictory. Here, we describe an experimental system to systematically examine these aspects. Nude mice were xenografted with human HT29 or SW620 colorectal carcinoma (CRC) cells and ctDNA was analyzed by Q-PCR with highly specific and sensitive primer sets at different times post-graft. We could discriminate ctDNA from normal (murine) cells and from mutated and non-mutated tumor (human) cells by using species-specific KRAS or PSAT1 primers and by assessing the presence of the BRAF V600E mutation. The concentration of human (mutated and non-mutated) ctDNA increased significantly with tumor growth. Conversely, and differently from previous studies, low, constant level of mouse ctDNA was observed, thus facilitating the study of mutated and non-mutated tumor derived ctDNA. Finally, analysis of ctDNA fragmentation confirmed the predominance of low-size fragments among tumor ctDNA from mice with bigger tumors. Higher ctDNA fragmentation was also observed in plasma samples from three metastatic CRC patients in comparison to healthy individuals. Our data confirm the predominance of mononucleosome-derived fragments in plasma from xenografted animals and, as a consequence, of apoptosis as a source of ctDNA, in particular for tumor-derived ctDNA. Altogether, our results suggest that ctDNA features vary during CRC tumor development and our experimental system might be a useful tool to follow such variations.

Indexed as

AnimalsCell Line, TumorColorectal NeoplasmsDNA FragmentationDNA, NeoplasmDNA PrimersFemaleHumansMiceMice, NudeMutationNeoplasm TransplantationPlasmaPolymerase Chain ReactionSerumSpecies SpecificityDNA, NeoplasmDNA Primers

Identifiers

PMID20494973
PMCPMC2952865
OpenAlexW1989498651

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.