ArticleMicrobial cell factories2010
High yield expression of an AHL-lactonase from Bacillus sp. B546 in Pichia pastoris and its application to reduce Aeromonas hydrophila mortality in aquaculture.
Article in Microbial cell factories, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed, 119 citations in OpenAlex.
- Quorum-quenching for bacterial pathogen control and health management in aquaculture: mechanisms, applications, current status and future prospects.Frontiers in systems biology · 2026Review
- Quorum quenching by endophytic Bacillus cereus AL1: a lactonase-based anti-virulence strategy against Pseudomonas aeruginosa.BMC microbiology · 2025Article
- Molecular Aspects of the Functioning of Pathogenic Bacteria Biofilm Based onInternational journal of molecular sciences · 2024Review
- Investigating quorum-quenching marine bacilli as potential biocontrol agents for protection of shrimps against Early Mortality Syndrome (EMS).Scientific reports · 2023Article
- The antivirulence activity, transcriptomics of EGCG and its protective effects on zebrafish infected byFrontiers in cellular and infection microbiology · 2023Article
- Cloning and characterization of Aiia, an acylhomoserine lactonase from Bacillus cereus RC1 to control soft rot causing pathogen Lelliottia amnigena RCE.Archives of microbiology · 2022Article
- Optimization of cultural conditions for enhancement of anti-quorum sensing potential in the probiotic strain3 Biotech · 2022Article
- Quorum Quenching Strains Isolated from the Microbiota of Sea Anemones and Holothurians AttenuateMicroorganisms · 2022Article
- Novel Quorum Quenching YtnP Lactonase FromFrontiers in microbiology · 2022Article
- Article
- Attenuation ofAntibiotics (Basel, Switzerland) · 2021Article
- The Roles of Microbial Cell-Cell Chemical Communication Systems in the Modulation of Antimicrobial Resistance.Antibiotics (Basel, Switzerland) · 2020Review
- Heterologous expression of AHL lactonase AiiK by Lactobacillus casei MCJΔ1 with great quorum quenching ability against Aeromonas hydrophila AH-1 and AH-4.Microbial cell factories · 2020Article
- Whole-Genome Sequencing Analysis of Quorum Quenching Bacterial StrainInternational journal of molecular sciences · 2020Article
- Engineering acyl-homoserine lactone-interfering enzymes toward bacterial control.The Journal of biological chemistry · 2020Review
- Mechanisms and the role of probiotic Bacillus in mitigating fish pathogens in aquaculture.Fish physiology and biochemistry · 2020Review
- The Quorum Quenching Bacterium Bacillus licheniformis T-1 Protects Zebrafish against Aeromonas hydrophila Infection.Probiotics and antimicrobial proteins · 2020Article
- Recombinant N-acyl homoserine lactone-Lactonase AiiAMarine drugs · 2019Article
- A Quorum-Sensing Inhibitor Strain ofMarine drugs · 2019Article
- Stenotrophomonas maltophilia AHL-Degrading Strains Isolated from Marine Invertebrate Microbiota Attenuate the Virulence of Pectobacterium carotovorum and Vibrio coralliilyticus.Marine biotechnology (New York, N.Y.) · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAeromonas hydrophila is a serious pathogen and can cause hemorrhagic septicemia in fish. To control this disease, antibiotics and chemicals are widely used which can consequently result in "superbugs" and chemical accumulation in the food chain. Though vaccine against A. hydrophila is available, its use is limited due to multiple serotypes of this pathogen and problems of safety and efficacy. Another problem with vaccination is the ability to apply it to small fish especially in high numbers. In this study, we tried a new way to attenuate the A. hydrophila infection by using a quorum quenching strategy with a recombinant AHL-lactonase expressed in Pichia pastoris.
resultsThe AHL-lactonase (AiiAB546) from Bacillus sp. B546 was produced extracellularly in P. pastoris with a yield of 3,558.4 +/- 81.3 U/mL in a 3.7-L fermenter when using 3-oxo-C8-HSL as the substrate. After purification with a HiTrap Q Sepharose column, the recombinant homogenous protein showed a band of 33.6 kDa on SDS-PAGE, higher than the calculated molecular mass (28.14 kDa). Deglycosylation of AiiAB546 with Endo H confirmed the occurrence of N-glycosylation. The purified recombinant AiiAB546 showed optimal activity at pH 8.0 and 20 degrees C, exhibited excellent stability at pH 8.0-12.0 and thermal stability at 70 degrees C, was firstly confirmed to be significantly protease-resistant, and had wide substrate specificity. In application test, when co-injected with A. hydrophila in common carp, recombinant AiiAB546 decreased the mortality rate and delayed the mortality time of fish.
conclusionsOur results not only indicate the possibility of mass-production of AHL-lactonase at low cost, but also open up a promising foreground of application of AHL-lactonase in fish to control A. hydrophila disease by regulating its virulence. To our knowledge, this is the first report on heterologous expression of AHL-lactonase in P. pastoris and attenuating A. hydrophila virulence by co-injection with AHL-lactonase.
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